Haemoglobin modulates salicylate and jasmonate/ethylene-mediated resistance mechanisms against pathogens
| Autor: | Luis A. J. Mur, Julien Mandon, Frans J. M. Harren, Anushen Sivakumaran, Kim H. Hebelstrup, Simona M. Cristescu |
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| Rok vydání: | 2012 |
| Předmět: |
Hypersensitive response
Botrytis cinerea haemoglobin hypersensitive response nitric oxide Pseudomomas syringae salicylic acid Ethylene hypersensitive response Physiology salicylic acid Arabidopsis Down-Regulation Pseudomonas syringae Plant Science Cyclopentanes Nitric Oxide Microbiology Nitric oxide chemistry.chemical_compound Hemoglobins Botrytis cinerea Plant Growth Regulators Gene Expression Regulation Plant nitric oxide Plant Immunity Jasmonate Oxylipins Plant Diseases biology Arabidopsis Proteins fungi food and beverages Ethylenes biology.organism_classification Plants Genetically Modified haemoglobin Plant Leaves Phenotype chemistry Host-Pathogen Interactions Mutation Botrytis Molecular and Laser Physics Salicylic Acid Pseudomomas syringae Salicylic acid Research Paper |
| Zdroj: | Journal of Experimental Botany, 63, 4375-4387 Mur, L A J, Sivakumaran, A, Mandon, J, Cristescu, S M, Harren, F J M & Hebelstrup, K 2012, ' Haemoglobin modulates salicylate and jasmonate/ethylene-mediated resistance mechanisms against pathogens ', Journal of Experimental Botany, vol. 63, no. 12, pp. 4375-4387 . https://doi.org/10.1093/jxb/ers116 Journal of Experimental Botany, 63, 12, pp. 4375-4387 Journal of Experimental Botany |
| ISSN: | 0022-0957 |
| DOI: | 10.1093/jxb/ers116 |
| Popis: | Nitric oxide (NO) plays a role in defence against hemibiotrophic pathogens mediated by salicylate (SA) and also necrotrophic pathogens influenced by jasmonate/ethylene (JA/Et). This study examined how NO-oxidizing haemoglobins (Hb) encoded by GLB1, GLB2, and GLB3 in Arabidopsis could influence both defence pathways. The impact of Hb on responses to the hemibiotrophic Pseudomonas syringae pathovar tomato (Pst) AvrRpm1 and the necrotrophic Botrytis cinerea were investigated using glb1, glb2, and glb3 mutant lines and also CaMV 35S GLB1 and GLB2 overexpression lines. In glb1, but not glb2 and glb3, increased resistance was observed to both pathogens but was compromised in the 35S-GLB1. A quantum cascade laser-based sensor measured elevated NO production in glb1 infected with Pst AvrRpm1 and B. cinerea, which was reduced in 35S-GLB1 compared to Col-0. SA accumulation was increased in glb1 and reduced in 35S-GLB1 compared to controls following attack by Pst AvrRpm1. Similarly, JA and Et levels were increased in glb1 but decreased in 35S-GLB1 in response to attack by B. cinerea. Quantitative PCR assays indicated reduced GLB1 expression during challenge with either pathogen, thus this may elevate NO concentration and promote a wide-ranging defence against pathogens. |
| Databáze: | OpenAIRE |
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