Unfolding Features of Bovine Testicular Hyaluronidase Studied by Fluorescence Spectroscopy and Fourier Transformed Infrared Spectroscopy
| Autor: | Nina Pan, Kai Tang, Xiaoqiang Cai, Guolin Zou |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Protein Folding Conformational change Sociology and Political Science Clinical Biochemistry Hyaluronoglucosaminidase Infrared spectroscopy Biochemistry Protein Structure Secondary Fluorescence spectroscopy Spectroscopy Fourier Transform Infrared Testis Animals Fourier transform infrared spectroscopy Spectroscopy Chemistry technology industry and agriculture Fluorescence Clinical Psychology Crystallography Spectrometry Fluorescence biological sciences Thermodynamics Cattle Protein folding Fluorescence cross-correlation spectroscopy Law Social Sciences (miscellaneous) |
| Zdroj: | Journal of Fluorescence. 15:841-847 |
| ISSN: | 1573-4994 1053-0509 |
| DOI: | 10.1007/s10895-005-0011-6 |
| Popis: | Chemical unfolding of bovine testicular hyaluronidase (HAase) has been studied by fluorescence spectroscopy and Fourier transformed infrared spectroscopy (FTIR). Thermodynamic parameters were determined for unfolding HAase from changes in the intrinsic fluorescence emission intensity and the formations of several possible unfolding intermediates have been identified. This was further confirmed by representation of fluorescence data in terms of 'phase diagram'. The secondary structures of HAase have been assigned and semiquantitatively estimated from the FTIR. The occurrence of conformational change during chemical unfolding as judged by fluorescence and FTIR spectroscopy indicated that the unfolding of HAase may not follow the typical two-state model. |
| Databáze: | OpenAIRE |
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