Hydrogen production by Enterobacter sp. LBTM 2 using sugarcane bagasse hemicellulose hydrolysate and a synthetic substrate: understanding and controlling toxicity
| Autor: | Bruno Eduardo Lobo Baeta, Jose Augusto Zorel, Flaviane Cristina Silva, Ivon M. Campos, Marina Caldeira Tonucci, Marília Bueno da Silva Menegatto, Sergio Francisco de Aquino, Oscar Fernando Herrera Adarme, Silvana de Queiroz Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Formic acid
Science Enterobacter Lignocellulosic biomass Xylose Furfural chemistry.chemical_compound Acetic acid Polysaccharides Organic chemistry Biohydrogen activated carbon Cellulose fermentation Hydrogen production biorefinery Multidisciplinary Hydrolysis xylose Saccharum acetic acid chemistry molecularly imprinted polymers Fermentation Hydrogen |
| Zdroj: | Anais da Academia Brasileira de Ciências, Volume: 93 Supplement 3, Article number: e20201679, Published: 27 SEP 2021 Anais da Academia Brasileira de Ciências, Vol 93, Iss suppl 3 (2021) Anais da Academia Brasileira de Ciências v.93 suppl.3 2021 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC |
| Popis: | Sugars released by thermochemical pretreatment of lignocellulosic biomass are possible substrate for hydrogen production. However, the major drawback for bacterial fermentation is the toxicity of weak acids and furan derivatives normally present in such substrate. This study aimed to investigate the metabolism involved in hydrogen production by the isolate Enterobacter LBTM2 using 10, 20 and 30-fold diluted synthetic (SH) and sugarcane bagasse hemicellulose (SBH) hydrolysates. In addition, the effects of acetic acid, formic acid and furfural on the bacterial metabolism, as well as detoxification of SBH with activated carbon and molecularly imprinted polymers on the hydrogen production were assessed. The results showed the best hydrogen yield was 0.46 mmol H2/mmol sugar for 20-times diluted SH, which was 2.3-times higher than obtained in SBH experiments. Bacterial growth and hydrogen production were negatively affected by 0.8 g/L of acetic acid when added alone, but were totally inhibited when formic acid (0.4 g/L) and furfural (0.3 g/L) were also supplied. However the maximum hydrogen production of SBH20 has duplicated when 3% of powdered activated carbon was added to the SBH experiment. The results presented herein can be helpful in understanding the bottlenecks in biohydrogen production and could contribute towards development of lignocellulosic biorefinery. |
| Databáze: | OpenAIRE |
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