High-performance liquid chromatographic assay with fluorescence detection for the simultaneous measurement of carboxylate and lactone forms of irinotecan and three metabolites in human plasma
| Autor: | Suzan K. Hanna, Katrin Fricke, Kristine R. Crews, Thandranese S. Owens, Helen M. Dodds |
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| Rok vydání: | 2003 |
| Předmět: |
Clinical Biochemistry
Carboxylic Acids SN-38 Irinotecan Sensitivity and Specificity Biochemistry Analytical Chemistry Lactones chemistry.chemical_compound Humans Carboxylate Acetonitrile Chromatography High Pressure Liquid chemistry.chemical_classification Detection limit Chromatography Reproducibility of Results Cell Biology General Medicine Antineoplastic Agents Phytogenic Spectrometry Fluorescence Linear range chemistry Calibration Camptothecin Glucuronide Ammonium acetate Lactone |
| Zdroj: | Journal of Chromatography B. 788:65-74 |
| ISSN: | 1570-0232 |
| Popis: | Irinotecan (CPT-11), a camptothecin analog, is metabolized to SN-38, an active topoisomerase I inhibitor, and inactive metabolites, including APC and SN-38 glucuronide (SN-38G). A high-performance liquid chromatographic assay method to simultaneously measure the lactone and carboxylate forms of CPT-11, SN-38, SN-38G, and APC in human plasma was developed. Chromatography was accomplished with a reversed-phase C8 column and fluorescence detection. A gradient mobile phase system was used. The buffer for mobile phase A consisted of 0.75 M ammonium acetate, 5 mM tetrabutylammonium phosphate (pH 6.0), and acetonitrile (86:14, v/v). The buffer for mobile phase B was identical to mobile phase A with the exception of the concentration (50:50, v/v). Precipitation of plasma proteins was performed with cold methanol. The linear range of detection of the lactone and carboxylate forms of SN-38, SN-38G, and APC was 2–25 ng/ml, and 5–300 ng/ml for CPT-11. The limit of quantitation for the analytes ranged from 0.5 to 5 ng/ml. Analysis of patients’ plasma samples obtained before and after CPT-11 administration showed that the assay is suitable for measuring lactone and carboxylate forms of CPT-11, SN-38, SN-38G, and APC in clinical studies. |
| Databáze: | OpenAIRE |
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