Colorimetric Determination of Citrulline Residues in Proteins
| Autor: | William L. Epstein, Kimie Fukuyama, Yusuke Yoshizawa, Sinfu Tzeng, Kiyoshi Sugawara |
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| Rok vydání: | 1998 |
| Předmět: |
Biophysics
Buffers Ferric Compounds Biochemistry Chloride chemistry.chemical_compound Ribonucleases Chlorides medicine Citrulline Animals Amino Acids Molecular Biology chemistry.chemical_classification Chromatography Epidermis (botany) Kunitz STI protease inhibitor Chemistry Proteins Reproducibility of Results Sulfuric acid Cell Biology Sulfuric Acids Molar absorptivity Rats Dithiothreitol Enzyme Animals Newborn Ferric Colorimetry Indicators and Reagents Epidermis medicine.drug |
| Zdroj: | Analytical Biochemistry. 265:92-96 |
| ISSN: | 0003-2697 |
| DOI: | 10.1006/abio.1998.2925 |
| Popis: | A method is described for the direct colorimetric determination of citrulline residues in proteins based on the reaction with diacetylmonoxime in the presence of lower concentrations of sulfuric acid. The reduced sensitivity due to the lower acid concentration was overcome by the addition of ferric chloride which also contributed to the color stabilization. Insoluble proteins or proteins resulting in turbidity can be analyzed following partial hydrolysis of those with enzyme or acid. The molar absorption coefficient (ϵ) for citrulline at 464 nm was 2.8 × 10 4 . The method enabled us to determine low levels of protein-bound citrulline which are beyond the limitations of conventional methods using an amino acid analyzer. We determined the citrulline contents in the cornified cells of the epidermis of newborn rats, as well as soybean trypsin inhibitor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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