Telomerase RNAs in land plants
| Autor: | Martina Dvořáčková, Jana Fulnečková, Šimon Dobias, Vratislav Peška, Dagmar Zachová, Agata Magdalena Kilar, Jiří Fajkus, Petr Fajkus, Jason Sims, Ivona Nečasová, Eva Sýkorová, Michal Závodník, Miloslava Fojtová |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Telomerase endocrine system animal structures Protein subunit Arabidopsis Asparagales 01 natural sciences Allium Evolution Molecular 03 medical and health sciences chemistry.chemical_compound Genetics RNA and RNA-protein complexes Arabidopsis thaliana Humans Telomerase reverse transcriptase Phylogeny 030304 developmental biology 0303 health sciences biology fungi RNA food and beverages Telomere biology.organism_classification chemistry Embryophyta DNA hormones hormone substitutes and hormone antagonists Genome Plant 010606 plant biology & botany |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| DOI: | 10.1093/nar/gkz695 |
| Popis: | To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt) allowed us to identify TRs in transcriptomic data of representative species of the Allium genus. Orthologous TRs were then identified in Asparagales plants harbouring telomere DNA composed of TTAGGG (human type) or TTTAGGG (Arabidopsis-type) repeats. Further, we identified TRs across the land plant phylogeny, including common model plants, crop plants, and plants with unusual telomeres. Several lines of functional testing demonstrate the templating telomerase function of the identified TRs and disprove a functionality of the only previously reported plant telomerase RNA in Arabidopsis thaliana. Importantly, our results change the existing paradigm in plant telomere biology which has been based on the existence of a relatively conserved telomerase reverse transcriptase subunit (TERT) associating with highly divergent TRs even between closely related plant taxa. The finding of a monophyletic origin of genuine TRs across land plants opens the possibility to identify TRs directly in transcriptomic or genomic data and/or predict telomere sequences synthesized according to the respective TR template region. |
| Databáze: | OpenAIRE |
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