Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages

Autor: Patrícia Sampaio Tavares Veras, Cintia Figueiredo de Araújo, Luiz Antonio Rodrigues de Freitas, Andre Cronemberger-Andrade, Cláudio P. Figueiras, Viviane Costa Junqueira Rocha, Pablo Rafael Silveira Oliveira, Mariana da Cruz Borges-Silva, Lain Pontes-de-Carvalho, Luciana S Aragão-França
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Células dendrítica mielóide
medicine.medical_treatment
Pathogenesis
Pathology and Laboratory Medicine
Mice
Medicine and Health Sciences
Leishmaniasis
Cells
Cultured
Ratos
Protozoans
Leishmania
Mice
Inbred BALB C
biology
medicine.diagnostic_test
lcsh:Public aspects of medicine
Cell biology
Infectious Diseases
Cytokine
Host-Pathogen Interactions
Infecção
Research Article
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
Immunology
Proinflammatory cytokine
Flow cytometry
Immune Activation
Extracellular Vesicles
Immune system
Células
Antigen
medicine
Animals
Macrófagos
MHC class II
Macrophages
Animais
Immunity
Organisms
Public Health
Environmental and Occupational Health
Biology and Life Sciences
lcsh:RA1-1270
biology.organism_classification
Parasitic Protozoans
Apoptosis
Mastócitos
biology.protein
Leishmania amazonensis
Zdroj: PLoS Neglected Tropical Diseases, Vol 8, Iss 9, p e3161 (2014)
PLoS Neglected Tropical Diseases
Repositório Institucional da FIOCRUZ (ARCA)
Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
ISSN: 1935-2735
1935-2727
Popis: Background Extracellular vesicles (EVs) are structures with phospholipid bilayer membranes and 100–1000 nm diameters. These vesicles are released from cells upon activation of surface receptors and/or apoptosis. The production of EVs by dendritic cells, mast cells, macrophages, and B and T lymphocytes has been extensively reported in the literature. EVs may express MHC class II and other membrane surface molecules and carry antigens. The aim of this study was to investigate the role of EVs from Leishmania-infected macrophages as immune modulatory particles. Methodology/Principal Findings In this work it was shown that BALB/c mouse bone marrow-derived macrophages, either infected in vitro with Leishmania amazonensis or left uninfected, release comparable amounts of 50–300 nm-diameter extracellular vesicles (EVs). The EVs were characterized by flow cytometry and electron microscopy. The incubation of naïve macrophages with these EVs for 48 hours led to a statistically significant increase in the production of the cytokines IL-12, IL-1β, and TNF-α. Conclusions/Significance EVs derived from macrophages infected with L. amazonensis induce other macrophages, which in vivo could be bystander cells, to produce the proinflammatory cytokines IL-12, IL-1β and TNF-α. This could contribute both to modulate the immune system in favor of a Th1 immune response and to the elimination of the Leishmania, leading, therefore, to the control the infection.
Author Summary Leishmaniases are a group of diseases—each one individually called leishmaniasis—that are caused by the protozoan Leishmania. They affect millions of people and thousands of dogs in tropical and mediterranean countries. Macrophages are the main cellular hosts of Leishmania in the mammalian host, where it is an obligatorily intracellular parasite. In this work, it is shown that mouse bone marrow-derived macrophages, when infected in vitro with Leishmania, release small (no larger than 300 nm) extracellular vesicles (EVs), in the same way as uninfected macrophages. The EVs from the infected macrophages, however, induce in other macrophages the production of some cell hormones, named cytokines, which are involved with protection of the macrophage against infection and with the development of a protective immune response against the parasite.
Databáze: OpenAIRE
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