53BP1 represses mitotic catastrophe in syncytia elicited by the HIV-1 envelope
| Autor: | Guido Kroemer, Claire Séror, Sara Sepe, Frédéric Subra, F Brottes, Luciana Chessa, Syed Qasim Raza, Roberta Nardacci, M-L Gougeon, F Del Nonno, Mauro Piacentini, J-L Perfettini, Héla Saïdi, Alessandra Amendola, A D'Incecco |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Adult Settore BIO/06 pml DNA damage Mitosis Cell Cycle Proteins Ataxia Telangiectasia Mutated Proteins Protein Serine-Threonine Kinases Biology Giant Cells Karyogamy env Gene Products atm aids apoptosis dna damage response Humans Phosphorylation Molecular Biology Mitotic catastrophe Syncytium Kinase Tumor Suppressor Proteins env Gene Products Human Immunodeficiency Virus Intracellular Signaling Peptides and Proteins virus diseases Cell Biology Protein-Serine-Threonine Kinases Immunohistochemistry Cell biology DNA-Binding Proteins Apoptosis Hela Cells HIV-1 RNA Interference DNA Damage Tumor Suppressor p53-Binding Protein 1 Human Immunodeficiency Virus |
| Popis: | p53 binding protein-1 (53BP1) participates in checkpoint signaling during the DNA damage response (DDR) and during mitosis. In this study we report that 53BP1 aggregates in nuclear foci within syncytia elicited by the human immunodeficiency virus (HIV)-1 envelope. 53BP1 aggregation occurs as a consequence of nuclear fusion (karyogamy (KG)). It colocalizes partially with the promyelomonocytic leukemia protein (PML), and the ataxia telangiectasia mutated kinase (ATM), the two components of the DDR that mediate apoptosis induced by the HIV-1 envelope. ATM-dependent phosphorylation of 53BP1 on serines 25 and 1778 (53BP1S25P and 53BP1S1778P) occurs at these DNA damage foci. 53BP1S25P was also detected in syncytia present in the lymph nodes or frontal brain sections from HIV-1-infected carriers, as well as in peripheral blood mononucleated cells from HIV-1-infected individuals, correlating with viral load. Knockdown of 53BP1 caused HIV-1 envelope-induced syncytia to enter abnormal mitoses, leading to their selective destruction through mitochondrion-dependent and caspase-dependent pathways. In conclusion, depletion of 53BP1 triggers the demise of HIV-1-elicited syncytia through mitotic catastrophe. |
| Databáze: | OpenAIRE |
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