Ergosterol gene expression in wild-type and ergosterol-deficient mutants ofCandidaalbicans
| Autor: | James A. Eckstein, C. A. Pierson, Robert J. Barbuch, Martin Bard |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Transcription
Genetic Cytochrome Mutant Fungal Proteins chemistry.chemical_compound Ergosterol Gene Expression Regulation Fungal Candida albicans Cytochrome b5 polycyclic compounds RNA Messenger biology Wild type Cytochrome P450 reductase RNA Fungal General Medicine biology.organism_classification Sterol Culture Media Infectious Diseases chemistry Biochemistry Mutation biology.protein lipids (amino acids peptides and proteins) |
| Zdroj: | Medical Mycology. 42:385-389 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.1080/13693780410001712016 |
| Popis: | The ergosterol pathway is the major target of the azole antifungals. We have developed a panel of five viable ergosterol biosynthetic mutants (erg2, erg3, erg6, erg11 and erg24) and have performed Northern analyses to study transcriptional regulation using probes to four ergosterol biosynthetic genes (ERG2, ERG7, ERG11 and ERG25), as well as probes to two additional genes encoding ergosterol cytochrome coenzymes (CYB5 and NCP1). ERG11, which encodes the sterol 14-demethylase, the direct target of the azole antifungals, was the most up-regulated gene followed by ERG7 and ERG25. Transcription of the four ergosterol genes was most up-regulated in erg24 and erg6 mutant backgrounds, deficient in C-14 reductase and the C-24 sterol transmethylase, respectively. Unexpectedly, we also found that the two cytochrome genes, CYB5 encoding cytochrome b5 and NCP1 encoding the cytochrome P450 reductase, were not regulated markedly different from wild-type in the erg2, erg3, erg6, erg11 and erg24 strains of Candida albicans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|