Different methodologies for evaluating the effect of clopidogrel on platelet function in high-risk coronary artery disease patients
| Autor: | Rossella Marcucci, Rita Paniccia, Domenico Prisco, Anna Maria Gori, Gian Franco Gensini, D. Antoniucci, Rosanna Abbate, Emilia Antonucci, Cristina Giglioli |
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| Rok vydání: | 2007 |
| Předmět: |
Blood Platelets
Male Light transmission medicine.medical_specialty Ticlopidine medicine.medical_treatment Coronary Artery Disease Coronary artery disease P2Y12 Risk Factors Internal medicine medicine Humans Platelet Phosphorylation Aged business.industry Percutaneous coronary intervention Hematology Middle Aged Clopidogrel medicine.disease Flow Cytometry Cardiology Platelet aggregation inhibitor Female business Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Journal of thrombosis and haemostasis : JTH. 5(9) |
| ISSN: | 1538-7933 |
| Popis: | Summary. Background: Two point-of-care (POC) systems have been recently proposed as rapid tools with which to evaluate residual platelet reactivity (RPR) in coronary artery disease (CAD) patients. Objectives and Methods: We compared Platelet Function Analyzer-100 (PFA-100) closure times (CTs) by collagen/adenosine 5´-diphosphate (ADP) (C/ADP CT) cartridge and the VerifyNow P2Y12 Assay (VerifyNow) with light transmission aggregation (LTA) induced by 2 and 10 μmol L–1 ADP in 1267 CAD patients on dual antiplatelet therapy who underwent percutaneous coronary intervention. We also performed the vasodilator-stimulated phosphoprotein (VASP) phosphorylation assay by cytofluorimetric analysis in a subgroup of 115 patients. Results: Cut-off values for identifying RPR were: ≥ 54% and ≥ 66% for LTA induced by 2 and 10 μmol L–1 ADP respectively, and ≥ 264 P2Y12 Reaction Units (PRU) for VerifyNow. The cut-off for PFA-100 C/ADP CT was ≥ 68 s. RPR was detected in 25.1% of patients by 2 μmol L–1 ADP-induced LTA (ADP-LTA), in 23.2% by 10 μmol L–1 ADP-LTA, in 24.4% by PFA-100, and in 24.7% by VerifyNow. PFA-100 results did not parallel those obtained with LTA. VerifyNow showed a significant correlation (ρ = 0.62, P |
| Databáze: | OpenAIRE |
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