An α-amylase inhibitor gene from Phaseolus coccineus encodes a protein with potential for control of coffee berry borer (Hypothenemus hampei)
| Autor: | Arnubio Valencia Jiménez, Railene A. Pereira, Maria Fátima Grossi-de-Sá, Osmundo Brilhante de Oliveira Neto, Maria Cristina Mattar da Silva, João Batista, E. L. Z. Figueira |
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| Rok vydání: | 2006 |
| Předmět: |
food.ingredient
Genetic Vectors Molecular Sequence Data Plant Science Horticulture Biology Coffee Biochemistry Gene product food Tobacco Botany Phaseolus coccineus Animals Amino Acid Sequence Amylase Cloning Molecular Enzyme Inhibitors Pest Control Biological Molecular Biology Gene Plant Proteins Phaseolus Cloning Base Sequence Protein primary structure food and beverages General Medicine Plants Genetically Modified Molecular biology Recombinant Proteins Genetically modified organism biology.protein Weevils alpha-Amylases Cotyledon Plasmids |
| Zdroj: | Phytochemistry. 67:2009-2016 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/j.phytochem.2006.06.029 |
| Popis: | Plant alpha-amylase inhibitors are proteins found in several plants, and play a key role in natural defenses. In this study, a gene encoding an alpha-amylase inhibitor, named alphaAI-Pc1, was isolated from cotyledons of Phaseolus coccineus. This inhibitor has an enhanced primary structure to P. vulgaris alpha-amylase inhibitors (alpha-AI1 and alpha-AI2). The alphaAI-Pc1 gene, constructed with the PHA-L phytohemaglutinin promoter, was introduced into tobacco plants, with its expression in regenerated (T0) and progeny (T1) transformant plants monitored by PCR amplification, enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis, respectively. Seed protein extracts from selected transformants reacted positively with a polyclonal antibody raised against alphaAI-1, while no reaction was observed with untransformed tobacco plants. Immunological assays showed that the alphaAI-Pc1 gene product represented up to 0.05% of total soluble proteins in T0 plants seeds. Furthermore, recombinant alphaAI-Pc1 expressed in tobacco plants was able to inhibit 65% of digestive H. hampei alpha-amylases. The data herein suggest that the protein encoded by the alphaAI-Pc1 gene has potential to be introduced into coffee plants in order to increase their resistance to the coffee berry borer. |
| Databáze: | OpenAIRE |
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