In vitro effect of gold and silver nanoparticles on human spermatozoa
| Autor: | Elena Moretti, Cesare Castellini, Cesare Giordano, Gaia Terzuoli, Tommaso Renieri, Francesca Iacoponi, Giulia Collodel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
inorganic chemicals Silver Cell Survival Urology Metal Nanoparticles Human spermatozoa Motility Semen In Vitro Techniques Biology Nanoparticles Gold Silver nanoparticle Andrology chemistry.chemical_compound Endocrinology Microscopy Electron Transmission In vivo mental disorders Scanning transmission electron microscopy NP localisation Humans Eosin Y Sperm viability health care economics and organizations Sperm motility Au- and Ag-NPs technology industry and agriculture General Medicine Anatomy respiratory system Spermatozoa Sperm chemistry Electron Probe Microanalysis |
| Popis: | The cytotoxicity of Au/Ag nanoparticles (NPs) on human spermatozoa was investigated in vitro. Semen from donors were incubated (37 °C, 60'-120') with 30, 60, 125, 250 and 500 μM Au/Ag-NPs. Sperm motility was evaluated following WHO guidelines; sperm viability was assessed with eosin Y test. Au-NPs were characterised and localised with field emission gun-based scanning transmission electron microscope/energy dispersive spectroscopy and transmission electron microscopy. Both tested NPs exerted a significant dose-dependent effect on motility and viability of human spermatozoa (P 0.001). Ag-NPs seem to show a slightly elevated toxicity although not significant (P 0.05). Au-NPs were localised in spermatozoa, whereas Ag-NPs were undetectable. In conclusion, Au-NPs and Ag-NPs do not appear to be harmful for human spermatozoa up to high concentrations (250-500 μM) that are probably difficult to reach in vivo. It is mandatory to explore the genotoxic effect of NPs in germ cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|