Combined treatment with ultrasound-targeted microbubble destruction technique and NM-aFGF-loaded PEG-nanoliposomes protects against diabetic cardiomyopathy-induced oxidative stress by activating the AKT/GSK-3β1/Nrf-2 pathway

Autor:	Ning-Wei Zhu, Meng-Jia Chen, Ming Zhang, Wei-Cheng Ma, Lei Zheng
Rok vydání:	2020
Předmět:	Male Diabetic Cardiomyopathies Chemistry Pharmaceutical Pharmaceutical Science RM1-950 02 engineering and technology medicine.disease_cause 030226 pharmacology & pharmacy nm-afgf Polyethylene Glycols Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Combined treatment Malondialdehyde Diabetic cardiomyopathy PEG ratio diabetic cardiomyopathy medicine Animals oxidative stress Ultrasonics Acidic Fibroblast Growth Factor Protein kinase B Glutathione Peroxidase Microbubbles Superoxide Dismutase Chemistry business.industry Ultrasound General Medicine 021001 nanoscience & nanotechnology medicine.disease Rats Liposomes utmd Cancer research Fibroblast Growth Factor 1 Nanoparticles Therapeutics. Pharmacology 0210 nano-technology business Oxidative stress Research Article
Zdroj:	Drug Delivery, Vol 27, Iss 1, Pp 938-952 (2020) Drug Delivery article-version (VoR) Version of Record
ISSN:	1521-0464 1071-7544
DOI:	10.1080/10717544.2020.1785052
Popis:	The present study sought to investigate the effect of non-mitogenic acidic fibroblast growth factor (NM-aFGF) loaded PEGylated nanoliposomes (NM-aFGF-PEG-lips) combined with the ultrasound-targeted microbubble destruction (UTMD) technique on modulating diabetic cardiomyopathy (DCM)and the mechanism involved. Animal studies showed that the diabetes mellitus (DM) group exhibited typical myocardial structural and functional changes of DCM. The indexes from the transthoracic echocardiography showed that the left ventricular function in the NM-aFGF-PEG-lips + UTMD group was significantly improved compared with the DM group. Histopathological observation further confirmed that the cardiomyocyte structural abnormalities and mitochondria ultrastructural changes were also significantly improved in the NM-aFGF-PEG-lips + UTMD group compared with DM group. The cardiac volume fraction (CVF) and apoptosis index in the NM-aFGF-PEG-lips + UTMD group decreased to 10.31 ± 0.76% and 2.16 ± 0.34, respectively, compared with those in the DM group (CVF = 21.4 ± 2.32, apoptosis index = 11.51 ± 1.24%). Moreover, we also found significantly increased superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity as well as clearly decreased lipid hydroperoxide levels and malondialdehyde (MDA) activity in the NM-aFGF-PEG-lips + UTMD group compared with those in the DM group (p
Databáze:	OpenAIRE
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