Lack of T-lymphocytosis and poor interferon gamma production in BAL fluid from HIV-negative immunocompetent patients with pulmonary non-tuberculous mycobacteriosis
| Autor: | Nadja Heurlin, Birger Christensson, Staffan Bergström, M. Christensson, Jan Andersson |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Microbiology (medical)
Lung Diseases Male Cellular immunity Lymphocytosis Neutrophils Lymphocyte T-Lymphocytes Population Mycobacterium Infections Nontuberculous Interferon-gamma Leukocyte Count HIV Seronegativity medicine Humans Interferon gamma education Aged Mycobacterium avium-intracellulare Infection Inflammation education.field_of_study General Immunology and Microbiology Interferon-gamma production medicine.diagnostic_test business.industry Interleukin-8 Granulocytosis General Medicine Middle Aged medicine.disease Infectious Diseases Bronchoalveolar lavage medicine.anatomical_structure Immunology Cytokines Female medicine.symptom business Bronchoalveolar Lavage Fluid Immunocompetence medicine.drug |
| Zdroj: | Scandinavian journal of infectious diseases. 30(4) |
| ISSN: | 0036-5548 |
| Popis: | Very little is known about the pathogenesis of pulmonary non-tuberculous mycobacteriosis in immunocompetent individuals. Local inflammatory response was assessed by examining bronchoalveolar lavage fluid from 13 HIV-negative patients (12 F) without known cell-mediated immunosuppression, aged 48-72 y (median age 60 y), with non-tuberculous lung mycobacteriosis. Macrophages, lymphocytes, polymorphonuclear neutrophils and eosinophils in bronchoalveolar lavage fluid were analysed morphologically, and the subsets of T-lymphocytes (CD3+, CD4+, CD8+), HLA-DR+, B-lymphocytes (CD19+) and CD16+/CD56+ cells (natural killer, NK cells) were analysed by flow cytometry. Interleukin-1 beta (IL-1beta), IL-2, IL-4, IL-6, IL-8, IL-10 and interferon-gamma (IFN-gamma) levels were assessed by ELISA. The total number of cells/ml was significantly higher in BAL fluid from the patients (median value=880 x 10(3)/ml) compared to six healthy controls (200 x 10(3)/ml). The polymorphonuclear neutrophil population was significantly increased in the patients both proportionally and in the count/ml. The proportion of macrophages was significantly reduced in the patients but not the count/ml. The count of lymphocytes/ml was significantly higher in the patients but the proportion of lymphocytes was unchanged. No significant difference was seen in the relative proportion of NK cells, B- or T-lymphocytes and HLA-DR+ compared to the healthy controls. The IL-1beta and IL-8 levels were significantly increased in the patients. No differences were seen between the patients and controls in the leukocyte or lymphocyte subsets in peripheral blood. The local inflammatory response in BAL fluid from the studied patients was characterized by granulocytosis, and increase in the IL-1beta and IL-8 levels. There was no specific T-cell response. |
| Databáze: | OpenAIRE |
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