Development of a three-dimensionally printed scaffold grafted with bone forming peptide-1 for enhanced bone regeneration with in vitro and in vivo evaluations
| Autor: | Haram Nah, Sang Jin Lee, Hyung Keun Kim, Xiang Yun Yin, Byoung-Hyun Min, Jong-Eun Won, Su A Park, Yoo Seob Shin, Chang-Hak Han, Chul-Ho Kim, Il Keun Kwon |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Scaffold Bone Regeneration Bone Morphogenetic Protein 7 02 engineering and technology Bone healing 010402 general chemistry Bone tissue 01 natural sciences Regenerative medicine Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Osteogenesis medicine Animals Humans Bone regeneration Cells Cultured Tissue Scaffolds Chemistry Regeneration (biology) Mesenchymal stem cell Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Peptide Fragments 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials medicine.anatomical_structure Printing Three-Dimensional Polycaprolactone Rabbits 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Colloid and Interface Science. 539:468-480 |
| ISSN: | 0021-9797 |
| DOI: | 10.1016/j.jcis.2018.12.097 |
| Popis: | Defects in bone are some of the most difficult injuries to treat. Biomimetic scaffolds represent a promising approach for successful bone tissue regeneration. In this study, a three-dimensional (3D) scaffold with osteo-inductive functionality was designed and assayed both in-vitro and in-vivo. Bone formation peptide-1 (BFP1), an osteo-promoting specific peptide, was covalently bound to a 3D printed polycaprolactone (PCL) scaffold using polydopamine (DOPA). The amount of BFP1 immobilized on the surface was found to increase depending on the BFP1 concentration of the loading solution. To observe the biological effects of the 3D scaffolds, human tonsil-derived mesenchymal stem cells (hTMSCs) were isolated. The cells were cultured on the scaffolds and observed to rapidly differentiate into osteoblast-like cells with osteo-promoting capabilities. The scaffolds were implanted in a rabbit calvarial defect model for 8 weeks and successfully stimulated both vessel and bone regeneration. Osteo-promoting 3D scaffolds may provide a safer and more efficient approach for bone repair and remodelling in regenerative medicine. |
| Databáze: | OpenAIRE |
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