Altered Gene Expression Profiles in Peripheral Blood Mononuclear Cells in Obese Subjects
| Autor: | Tao Tong, Jinhui Lee, Taesun Park, Kyungho Jang, Howard Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Leptin Male medicine.medical_specialty obesity Health (social science) Waist 030209 endocrinology & metabolism lcsh:TX341-641 CCL2 Peripheral blood mononuclear cell Body Mass Index 03 medical and health sciences Young Adult 0302 clinical medicine Physiology (medical) Internal medicine Gene expression medicine pbmc Animals Humans Gene lcsh:RC620-627 030109 nutrition & dietetics Adiponectin business.industry Waist-Hip Ratio Body Weight Middle Aged medicine.disease Obesity Rats lcsh:Nutritional diseases. Deficiency diseases Endocrinology Leukocytes Mononuclear gene expression Female business Transcriptome lcsh:Nutrition. Foods and food supply Research Article |
| Zdroj: | Obesity Facts, Vol 13, Iss 3, Pp 375-385 (2020) Obes Facts |
| ISSN: | 1662-4033 1662-4025 |
| Popis: | Introduction: Gene expression profiles in human peripheral blood mononuclear cells (PBMCs) may act as a useful tool to better understand obesity. We investigated gene expression levels in PMBCs for possible differences between obese and non-obese subjects (19–55 years) and evaluated correlations between gene expression in PBMCs and clinical obesity indices. Methods: Body weight, BMI, fat amount, fat percentage, waist/hip ratio, leptin, and adiponectin levels were determined in 30 obese and 20 non-obese subjects. Expression levels of 19 genes, which were differentially expressed by clinical obesity indices in the PBMCs of high fat-fed rats, were determined in their PBMCs using real-time PCR. Results: The expression of 9 of 19 previously selected genes was significantly correlated with one or more clinical obesity indices. Both TFEC and CCL2 expression were negatively correlated with BMI, fat amount, fat percentage, waist/hip ratio, and leptin concentration. Similarly, TNFAIP2, VCAN, ASSI, IRF1, and HK3 expression negatively correlated with some clinical obesity indices, such as TNFAIP2 for BMI, fat amount, fat percentage, and waist/hip ratio, VCAN for fat amount, fat percentage, and waist/hip ratio, ASS1 for BMI and fat amount, IRF1 for BMI, fat amount, and fat percentage, and HK3 for fat amount. In contrast, both TNF-α and LPL expression were positively correlated with waist/hip ratio. Conclusion: We identified 9 of 19 genes in human PBMCs that significantly correlated with one or more clinical obesity indices. Because these genes have a mechanistic basis for the development or progression of obesity and its metabolic derangements, they may help to determine possible underlying mechanisms for obesity. |
| Databáze: | OpenAIRE |
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