Identification of flgZ as a flagellar gene encoding a PilZ domain protein that regulates swimming motility and biofilm formation in Pseudomonas
| Autor: | Miguel Redondo-Nieto, Rafael Rivilla, Irene Baena, Francisco Martínez-Granero, Ana Navazo, Irene Martín-Martín, Marta Martín, Elena González De Heredia, Emma Barahona |
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| Přispěvatelé: | UAM. Departamento de Biología |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Bacterial Diseases
Operon Movement Protein domain Biophysics lcsh:Medicine Pseudomonas fluorescens Flagellum Microbiology Plant Microbiology Bacterial Proteins Microbial Physiology Pseudomonas Pseudomonas Infections lcsh:Science Biology Cyclic GMP Conserved Sequence Multidisciplinary biology Cell swimming lcsh:R Biofilm Bacteriology Flagellar Motility biology.organism_classification Biología y Biomedicina / Biología Cell biology Bacterial Pathogens Protein Structure Tertiary PilZ domain Cell Motility Infectious Diseases Flagella Genes Bacterial Biofilms biology.protein Medicine Diguanylate cyclase lcsh:Q Bacterial Biofilms Research Article Subcellular Fractions |
| Zdroj: | PLoS ONE, Vol 9, Iss 2, p e87608 (2014) PLoS ONE Biblos-e Archivo. Repositorio Institucional de la UAM instname |
| ISSN: | 1932-6203 |
| Popis: | Diguanylate cyclase and phosphodiesterase enzymatic activities control c-di-GMP levels modulating planktonic versus sessile lifestyle behavior in bacteria. The PilZ domain is described as a sensor of c-di-GMP intracellular levels and the proteins containing a PilZ domain represent the best studied class of c-di-GMP receptors forming part of the c-di-GMP signaling cascade. In P. fluorescens F113 we have found two diguanylate cyclases (WspR, SadC) and one phosphodiesterase (BifA) implicated in regulation of swimming motility and biofilm formation. Here we identify a flgZ gene located in a flagellar operon encoding a protein that contains a PilZ domain. Moreover, we show that FlgZ subcellular localization depends on the c-di-GMP intracellular levels. The overexpression analysis of flgZ in P. fluorescens F113 and P. putida KT2440 backgrounds reveal a participation of FlgZ in Pseudomonas swimming motility regulation. Besides, the epistasis of flgZ over wspR and bifA clearly shows that c-di-GMP intracellular levels produced by the enzymatic activity of the diguanylate cyclase WspR and the phosphodiesterase BifA regulates biofilm formation through FlgZ This work was funded by the Spanish Ministry of Economy and Competitivity grant BIO2012-31634, and by the Research Program MICROAMBIENTE-CM from Comunidad de Madrid |
| Databáze: | OpenAIRE |
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