White blood cell DNA adducts in a cohort of asthmatic children exposed to environmental tobacco smoke
| Autor: | Robert S. Kahn, Anthony C. Leonard, Glenn Talaska, Brenda L. Schumann, Stephen E. Wilson, Bruce P. Lanphear, Jane C. Khoury |
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| Rok vydání: | 2009 |
| Předmět: |
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Physiology Air filters Tobacco smoke White People Nicotine Cohort Studies 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Environmental health White blood cell DNA adduct medicine Leukocytes Humans 030212 general & internal medicine Child Cotinine African American Asthma Air filter business.industry Public Health Environmental and Occupational Health DNA adducts medicine.disease 3. Good health Black or African American medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Child Preschool Cohort Female Tobacco Smoke Pollution Original Article business medicine.drug ETS |
| Zdroj: | International Archives of Occupational and Environmental Health |
| ISSN: | 1432-1246 |
| Popis: | Purpose Exposure to environmental tobacco smoke (ETS) leads to molecular damage in the form of DNA adducts. While lung cancer risk is higher among African Americans compared to White Americans, a few studies have tested for racial differences in DNA adducts among children exposed to ETS. The purpose of this study was to test whether African American children have higher DNA adducts levels compared to White children adjusted for ETS exposure. Methods Data and biologic specimens were drawn from an existing cohort of 212 asthmatic children. These subjects participated in a 12-month ETS-reduction trial that employed HEPA air cleaners with active filter cartridges and sham filter cartridges. White blood cell (WBC) DNA was analyzed for DNA adducts using 32P-postlabeling. We assessed ETS exposure using a validated air nicotine dosimeter. We determined the independent relationship between African American race and DNA adduct levels adjusted for ETS exposure and air cleaner use. Results The mean age of the subjects was 8.4 years; 55% were African American. There was no difference in DNA adduct levels between African American and White children (11.8 vs. 11.2 adducts per 109 nucleotides, p = 0.86), despite slightly higher levels of air nicotine exposure (3.4 vs. 2.2 μg/m3, p = 0.14). African American children used their air cleaners less often than White children. We found that the best predictor of DNA adduct levels was the duration of air cleaner use (r = −0.133, p = 0.056). This association was independent of cartridge type. Conclusions We did not see differences in adduct levels by race even after accounting for the level of ETS exposure. However, there was a marginal inverse association between air cleaner use and adducts. Additional research is required to understand this phenomenon. |
| Databáze: | OpenAIRE |
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