Fast separation of enantiomers by capillary electrophoresis using a combination of two capillaries with different internal diameters
| Autor: | Jan Petr, Andrea Šebestová |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Chemistry 010401 analytical chemistry Clinical Biochemistry Analytical chemistry Electrophoresis Capillary Reproducibility of Results Model system Stereoisomerism 02 engineering and technology Derivative Naphthalenes 021001 nanoscience & nanotechnology Hydrogen phosphate 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Fructans Capillary electrophoresis Models Chemical Enantiomer 0210 nano-technology Isopropyl Cyclofructan 6 |
| Zdroj: | Electrophoresis. 38(24) |
| ISSN: | 1522-2683 |
| Popis: | The combination of capillaries with different internal diameters was used to accelerate the separation of enantiomers in capillary electrophoresis. Separation of R,S-1,1′-binaphthalene-2,2′-diyl hydrogen phosphate using isopropyl derivative of cyclofructan 6 was studied as a model system. The best separation conditions included 500 mM sodium borate pH 9.5 with 60 mM concentration of the chiral selector. Separation lasted approx. 1.5 min using the combination of 50 and 100 μm id capillaries of 9.7 cm and 22.9 cm, respectively. It allowed approx. 12-fold acceleration in comparison to the traditional long-end separation mainly due to the higher electroosmotic flow generated in the connected capillaries. |
| Databáze: | OpenAIRE |
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