NIN-like protein7 and PROTEOLYSIS6 functional interaction enhances tolerance to sucrose, ABA, and submergence
| Autor: | Beatriz Gayubas, José León, Álvaro Costa-Broseta, Mari-Cruz Castillo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Sucrose
Regular Issue AcademicSubjects/SCI01280 Physiology Ubiquitin-Protein Ligases Proteolysis Mutant Arabidopsis Plant Science Nitric oxide chemistry.chemical_compound Immersion Signaling and Response Genetics medicine Arabidopsis thaliana Abscisic acid Transcription factor Research Articles AcademicSubjects/SCI01270 biology medicine.diagnostic_test AcademicSubjects/SCI02288 Arabidopsis Proteins AcademicSubjects/SCI02287 AcademicSubjects/SCI02286 biology.organism_classification Cell biology Complementation chemistry Abscisic Acid Transcription Factors |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Plant Physiology Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | Nitrate (NO3) assimilation and signaling regulate plant growth through the relevant function of the transcription factor NIN-like Protein7 (NLP7). NO3 is also the main source for plants to produce nitric oxide (NO), which regulates growth and stress responses. NO-mediated regulation requires efficient sensing via the PROTEOLYSIS6 (PRT6)-mediated proteasome-triggered degradation of group VII of ethylene response transcription factors through the Cys/Arg N-degron pathway. The convergence of NO3 signaling and N-degron proteolysis on NO-mediated regulation remains largely unknown. Here, we investigated the functional interaction between NLP7 and PRT6 using Arabidopsis (Arabidopsis thaliana) double prt6 nlp7 mutant plants as well as complementation lines overexpressing NLP7 in different mutant genetic backgrounds. prt6 nlp7 mutant plants displayed several potentiated prt6 characteristic phenotypes, including slower vegetative growth, increased NO content, and diminished tolerance to abiotic stresses such as high-sucrose concentration, abscisic acid, and hypoxia–reoxygenation. Although NLP7 has an N-terminus that could be targeted by the N-degron proteolytic pathway, it was not a PRT6 substrate. The potential PRT6- and NO-regulated nucleocytoplasmic translocation of NLP7, which is likely modulated by posttranslational modifications, is proposed to act as a regulatory loop to control NO homeostasis and action. Nitrate signaling and N-degron-mediated proteolysis interact to regulate plant tolerance to abiotic stresses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|