Fish oil supplementation for two generations increases insulin sensitivity in rats
| Autor: | Rafael Herling Lambertucci, Júlia Aikawa, Angelo Rafael Carpinelli, Renata Gorjão, Carlos F Rodrigues, Luiz Claudio Fernandes, Ricardo K. Yamazaki, Amanda R. Martins, Jarlei Fiamoncini, Rui Curi, Sandro M. Hirabara, Alessandra Folador, Alice Cristina Rodrigues, M. S. Rocha, Tania Cristina Pithon-Curi |
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| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Glucose uptake Clinical Biochemistry Carbohydrate metabolism p38 Mitogen-Activated Protein Kinases Biochemistry FARMACOLOGIA Fish Oils Insulin resistance Internal medicine Fatty Acids Omega-3 medicine Animals Phosphorylation Rats Wistar Muscle Skeletal Molecular Biology Soleus muscle Nutrition and Dietetics biology Insulin Insulin tolerance test DNA Methylation Fish oil medicine.disease Rats Insulin receptor Endocrinology Dietary Supplements biology.protein Female Insulin Resistance Proto-Oncogene Proteins c-akt |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | We investigated the effect of fish oil supplementation for two consecutive generations on insulin sensitivity in rats. After the nursing period (21 days), female rats from the same prole were divided into two groups: (a) control group and (b) fish oil group. Female rats were supplemented with water (control) or fish oil at 1 g/kg body weight as a single bolus for 3 months. After this period, female rats were mated with male Wistar rats fed on a balanced chow diet (not supplemented). Female rats continued to receive supplementation throughout gestation and lactation periods. The same treatment was performed for the next two generations (G1 and G2). At 75 days of age, male offspring from G1 and G2 generations from both groups were used in the experiments. G1 rats did not present any difference with control rats. However, G2 rats presented reduction in glycemia and lipidemia and improvement in in vivo insulin sensitivity (model assessment of insulin resistance, insulin tolerance test) as well as in vitro insulin sensitivity in soleus muscle (glucose uptake and metabolism). This effect was associated with increased insulin-stimulated p38 MAP kinase phosphorylation and lower n-6/n-3 fatty acid ratio, but not with activation of proteins from insulin signaling (IR, IRS-1 and Akt). Global DNA methylation was decreased in liver but not in soleus muscle. These results suggest that long-term fish oil supplementation improves insulin sensitivity in association with increased insulin-stimulated p38 activation and decreased n-6:n-3 ratio in skeletal muscle and decreased global DNA methylation in liver. |
| Databáze: | OpenAIRE |
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