An Energy-Independent Pro-longevity Function of Triacylglycerol in Yeast

Autor: Xiaobo Li, Min Hao Kuo, Witawas Handee, Christoph Benning, Barry L. Williams, Pan Li, Xiexiong Deng, Kevin W. Hall
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Cancer Research
Aging
Hydrolases
Physiology
Yeast and Fungal Models
Mitochondrion
medicine.disease_cause
Biochemistry
chemistry.chemical_compound
Medicine and Health Sciences
Lipases
Genetics (clinical)
Energy-Producing Organelles
media_common
Mutation
biology
Organic Compounds
Fatty Acids
Monosaccharides
Longevity
Lipids
Enzymes
Mitochondria
Chemistry
Physical Sciences
Cellular Structures and Organelles
Research Article
Paraquat
Saccharomyces cerevisiae Proteins
lcsh:QH426-470
media_common.quotation_subject
Saccharomyces cerevisiae
Carbohydrates
Bioenergetics
Research and Analysis Methods
03 medical and health sciences
Saccharomyces
Model Organisms
Biosynthesis
Genetics
medicine
Diacylglycerol O-Acyltransferase
Molecular Biology
Ecology
Evolution
Behavior and Systematics
Triglycerides
Sirolimus
Organic Chemistry
Organisms
Fungi
Chemical Compounds
Biology and Life Sciences
Proteins
Metabolism
Cell Biology
Lipase
biology.organism_classification
Yeast
lcsh:Genetics
030104 developmental biology
Glucose
chemistry
Enzymology
Physiological Processes
Energy Metabolism
Organism Development
Function (biology)
Developmental Biology
Zdroj: PLoS Genetics, Vol 12, Iss 2, p e1005878 (2016)
PLoS Genetics
ISSN: 1553-7404
1553-7390
Popis: Intracellular triacylglycerol (TAG) is a ubiquitous energy storage lipid also involved in lipid homeostasis and signaling. Comparatively, little is known about TAG’s role in other cellular functions. Here we show a pro-longevity function of TAG in the budding yeast Saccharomyces cerevisiae. In yeast strains derived from natural and laboratory environments a correlation between high levels of TAG and longer chronological lifespan was observed. Increased TAG abundance through the deletion of TAG lipases prolonged chronological lifespan of laboratory strains, while diminishing TAG biosynthesis shortened lifespan without apparently affecting vegetative growth. TAG-mediated lifespan extension was independent of several other known stress response factors involved in chronological aging. Because both lifespan regulation and TAG metabolism are conserved, this cellular pro-longevity function of TAG may extend to other organisms.
Author Summary Triacylglycerol (TAG) is a ubiquitous lipid species well-known for its roles in storing surplus energy, providing insulation, and maintaining cellular lipid homeostasis. Here we present evidence for a novel pro-longevity function of TAG in the budding yeast, a model organism for aging research. Yeast cells that are genetically engineered to store more TAG live significantly longer without suffering obvious growth defects, whereas those lean cells that are depleted of TAG die early. Yeast strains isolated from the wild in general contain more fat and also display longer lifespan. One of the approaches taken here to force the increase of intracellular TAG is to delete lipases responsible for lipid hydrolysis. Energy extraction from TAG thus is unlikely an underlying cause of the observed lifespan extension. Our results are reminiscent of certain animal studies linking higher body fat to longer lifespan. Potential mechanisms for the connection of TAG and yeast lifespan regulation are discussed.
Databáze: OpenAIRE
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