Protective effect of vitamin E on sperm motility and oxidative stress in valproic acid treated rats
| Autor: | Tanise S. Pês, Viviane Mara Woehl, Bernardo Baldisserotto, Giovana M. Ourique, Maria A. Pavanato, Werner G. Glanzner, Etiane M.H. Saccol, Kátia Padilha Barreto, Sun Hee Schiefelbein, Paulo Bayard Dias Gonçalves |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Vitamin medicine.medical_specialty medicine.medical_treatment Biology Protective Agents Toxicology medicine.disease_cause Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Testis medicine Animals Vitamin E Testosterone Rats Wistar Spermatogenesis Sperm motility Epididymis Sperm Count Valproic Acid General Medicine Glutathione Rats Oxidative Stress 030104 developmental biology Endocrinology chemistry Sperm Motility Anticonvulsants Biological Assay lipids (amino acids peptides and proteins) Lipid Peroxidation Reproductive toxicity alpha-Tocopherol 030217 neurology & neurosurgery Oxidative stress Food Science |
| Zdroj: | Food and Chemical Toxicology. 95:159-167 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2016.07.011 |
| Popis: | Long-term administration of valproic acid (VPA) is known to promote reproductive impairment mediated by increase in testicular oxidative stress. Vitamin E (VitE) is a lipophilic antioxidant known to be essential for mammalian spermatogenesis. However, the capacity of this vitamin to abrogate the VPA-mediated oxidative stress has not yet been assessed. In the current study, we evaluated the protective effect of VitE on functional abnormalities related to VPA-induced oxidative stress in the male reproductive system. VPA (400 mg kg −1 ) was administered by gavage and VitE (50 mg kg −1 ) intraperitoneally to male Wistar rats for 28 days. Analysis of spermatozoa from the cauda epididymides was performed. The testes and epididymides were collected for measurement of oxidative stress biomarkers. Treatment with VPA induced a decrease in sperm motility accompanied by an increase in oxidative damage to lipids and proteins, depletion of reduced glutathione and a decrease in total reactive antioxidant potential on testes and epididymides. Co-administration of VitE restored the antioxidant potential and prevented oxidative damage on testes and epididymides, restoring sperm motility. Thus, VitE protects the reproductive system from the VPA-induced damage, suggesting that it may be a useful compound to minimize the reproductive impairment in patients requiring long-term treatment with VPA. |
| Databáze: | OpenAIRE |
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