A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)
| Autor: | Jing-Hao Jin, Xiao-Hui Feng, Jing-Gang Lv, Su-Yan Gao, Muhammad Ali, Liu-Jun Huang, Huai-Xia Zhang, Abid Khan, Zhen-Hui Gong, De-Xu Luo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phytophthora capsici Nicotiana benthamiana Acetates Plant Roots 01 natural sciences CaSBP12 lcsh:Chemistry pepper Gene Expression Regulation Plant Plant defense against herbivory lcsh:QH301-705.5 Spectroscopy Disease Resistance Plant Proteins biology food and beverages General Medicine Plants Genetically Modified defense-related genes Computer Science Applications Capsicum Salicylic Acid Phytophthora Transgene Cyclopentanes Article Catalysis Microbiology Inorganic Chemistry 03 medical and health sciences Pepper Gene silencing Gene family Oxylipins Physical and Theoretical Chemistry Molecular Biology Gene Plant Diseases Organic Chemistry fungi biology.organism_classification Plant Leaves 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Transcription Factors 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 1, p 48 (2018) International Journal of Molecular Sciences Volume 20 Issue 1 |
| ISSN: | 1422-0067 |
| Popis: | SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction and stress response. However, little is known about the SBP-box genes in pepper (CaSBP), especially in the process of Phytophthora capsici infection. In this study, a novel gene (CaSBP12) was selected from the CaSBP gene family, which was isolated from the pepper genome database in our previous study. The CaSBP12 gene was located in the nucleus of the cell and its silencing in the pepper plant enhanced the defense response against Phytophthora capsici infection. After inoculation with Phytophthora capsici, the root activity of the CaSBP12-silenced plants is compared to control plants, while malondialdehyde (MDA) content is compared viceversa. Additionally, the expression of defense related genes (CaPO1, CaSAR8.2, CaBPR1, and CaDEF1) in the silenced plants were induced to different degrees and the peak of CaSAR8.2 and CaBPR1 were higher than that of CaDEF1. The CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to Phytophthora capsici infection with higher EC (electrical conductivity) and MDA contents as compared to the wild-type. The relative expression of defense related genes (NbDEF, NbNPR1, NbPR1a, and NbPR1b) in transgenic and wild-type Nicotiana benthamiana plants were induced, especially the NbPR1a and NbPR1b. In conclusion, these results indicate that CaSBP12 gene negative regulates the defense response against Phytophthora capsici infection which suggests their potentially significant role in plant defense. To our knowledge, this is the first report on CaSBP gene which negative regulate defense response. |
| Databáze: | OpenAIRE |
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