Immunophenotyping of acute myeloid leukaemia: relevance of analysing different lineage-associated markers
| Autor: | Enrico Panzini, D. Pasqualetti, M. Lopez, Francesco Lo Coco, Roberto Latagliata, Bruno Monarca, Alfonso Gentile, Giulio De Rossi |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Myeloid
Adult medicine.medical_specialty Adolescent medicine.drug_class Genetic Linkage CD33 Antigens Differentiation Myelomonocytic Acute Monoclonal antibody Group A CD19 Antibodies Immunophenotyping Antigen hemic and lymphatic diseases Internal medicine Monoclonal medicine Humans Antigens Preschool Child neoplasms Aged Hematology Leukemia biology Histocompatibility Testing Induction chemotherapy Antibodies Monoclonal Infant General Medicine Myelomonocytic Middle Aged Antigens Differentiation Biomarkers Child Preschool Leukemia Myeloid Acute Phenotype Differentiation Immunology biology.protein Settore MED/15 - Malattie del Sangue |
| Zdroj: | Blut. 58(5) |
| ISSN: | 0006-5242 |
| Popis: | The immunophenotype of 135 previously untreated patients with FAB defined acute myeloid leukaemia (AML) was studied at diagnosis. The panel of reagents included monoclonal antibodies (MoAb) recognising myeloid-associated determinants (CD11, CD13, CD14, CD33 and others) as well as MoAb directed towards lymphoid antigens (CD7, CD10, CD19) and TdT. The results indicate that CD13 and/or CD33 are consistently expressed in AML and only rarely in ALL blasts (131/135 + ve cases, versus 4/130 in ALL). Lymphoid antigen expression was rarely detected when CD10 and CD19 were investigated in AML (0.9% and 2% + ve cases, respectively), whereas significant positivities were found for TdT and CD7 (20% and 10% respectively). Concerning FAB subtypes, two new MoAb (LAM3 and LAM7) proved very useful in the specific recognition of AML with monocytic features. The phenotype CD13+ and/or CD33+, CD9+, HLA-DR- was found to be almost exclusive for M3 AML. The response to induction chemotherapy was analysed in CD7+ and in TdT+ patients. In the latter group a statistically significant lower response rate was found with respect to TdT-ve-AML patients. |
| Databáze: | OpenAIRE |
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