Exploring two-photon optogenetics beyond 1100 nm for specific and effective all-optical physiology
| Autor: | Albrecht Stroh, Wei Fan, Jan Döring, Isabelle Arnoux, Ignas Stasevičius, Hirofumi Watari, Ting Fu, Hendrik Backhaus |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
genetic structures Physiology 02 engineering and technology Optogenetics Article Photostimulation Molecular Physiology 03 medical and health sciences All optical Calcium imaging Two-photon excitation microscopy Cellular neuroscience medicine lcsh:Science Cellular Neuroscience Optical Imaging Physics Multidisciplinary Cortical neurons 021001 nanoscience & nanotechnology 030104 developmental biology Visual cortex medicine.anatomical_structure lcsh:Q 0210 nano-technology |
| Zdroj: | iScience, 24(3):102184 iScience iScience, Vol 24, Iss 3, Pp 102184-(2021) Iscience, Cambridge : Elsevier, 2021, vol. 24, iss. 3, art. no. 102184, p. [1-31] |
| ISSN: | 2589-0042 |
| Popis: | Summary Two-photon (2-P) all-optical approaches combine in vivo 2-P calcium imaging and 2-P optogenetic modulations. Here, firstly, we combined in vivo juxtacellular recordings and GCaMP6f-based 2-P calcium imaging in mouse visual cortex to tune our detection algorithm towards a 100% specific identification of action potential-related calcium transients. Secondly, we minimized photostimulation artifacts by using extended-wavelength-spectrum laser sources for optogenetic stimulation. We achieved artifact-free all-optical experiments performing optogenetic stimulation from 1100 nm to 1300 nm. Thirdly, we determined the spectral range for maximizing efficacy until 1300 nm. The rate of evoked transients in GCaMP6f/C1V1-co-expressing cortical neurons peaked already at 1100 nm. By refining spike detection and defining 1100 nm as the optimal wavelength for artifact-free and effective GCaMP6f/C1V1-based all-optical physiology, we increased the translational value of these approaches, e.g., for the development of network-based therapies. Graphical abstract Highlights • We developed an algorithm for 100 %-specific identification of AP-related calcium transients • Artifact-free all-optical experiments can be achieved from 1100 nm to 1300 nm • Efficacy of C1V1 excitation does not increase beyond 1100 nm until 1300 nm Optical Imaging; Molecular Physiology; Cellular Neuroscience |
| Databáze: | OpenAIRE |
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