Conversion of renewable substrates for biosurfactant production by Rhizopus arrhizus UCP 1607 and enhancing the removal of diesel oil from marine soil
Autor: | André Luiz Cabral Monteiro de Azevedo Santiago, Daniela S. Alviano, Milagre Americo Pele, Galba Maria de Campos-Takaki, Marcos Antônio Cavalcanti Luna, Edson Rodrigues Vieira, Dayana Montero Rodríguez, Celuta S. Alviano, Daylin Rubio Ribeaux, Rosileide F. S. Andrade, Adriana Ferreira Souza, Eliana Barreto-Bergter |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Pollutant biology lcsh:Biotechnology Factorial experiment biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Corn steep liquor 03 medical and health sciences Diesel fuel chemistry.chemical_compound 030104 developmental biology Bioremediation lcsh:Biology (General) chemistry Rhizopus lcsh:TP248.13-248.65 010608 biotechnology Glycerol Rhizopus arrhizus Food science lcsh:QH301-705.5 Biotechnology |
Zdroj: | Electronic Journal of Biotechnology, Vol 38, Iss, Pp 40-48 (2019) |
ISSN: | 0717-3458 |
Popis: | Background: The use of agro-industrial wastes to produce high value-added biomolecules such as biosurfactants is a promising approach for lowering the total costs of production. This study aimed to produce biosurfactants using Rhizopus arrhizus UCP 1607, with crude glycerol (CG) and corn steep liquor (CSL) as substrates. In addition, the biomolecule was characterized, and its efficiency in removing petroderivatives from marine soil was investigated. Results: A 22 factorial design was applied, and the best condition for producing the biosurfactant was determined in assay 4 (3% CG and 5% CSL). The biosurfactant reduced the surface tension of water from 72 to 28.8 mN/m and produced a yield of 1.74 g/L. The preliminary biochemical characterization showed that the biosurfactant consisted of proteins (38.0%), carbohydrates (35.4%), and lipids (5.5%). The compounds presented an anionic character, nontoxicity, and great stability for all conditions tested. The biomolecule displayed great ability in dispersing hydrophobic substrates in water, thereby resulting in 53.4 cm2 ODA. The best efficiency of the biosurfactant in removing the pollutant diesel oil from marine soil was 79.4%. Conclusions: This study demonstrated the ability of R. arrhizus UCP1607 to produce a low-cost biosurfactant characterized as a glycoprotein and its potential use in the bioremediation of the hydrophobic diesel oil pollutant in marine soil.How to cite: Pele MA, Ribeaux DR, Vieira ER, et al. Conversion of renewable substrates for biosurfactant production by Rhizopus arrhizus UCP 1607 and enhancing the removal of diesel oil from marine soil. Electron J Biotechnol 2019;38. https://doi.org/10.1016/j.ejbt.2018.12.003. Keywords: Agro-industrial wastes, Biomolecules, Biosurfactant, Corn steep liquor, Crude glycerol, Diesel oil, Marine soil, Mucoralean fungus, Removing hydrophobic compound, Rhizopus, Tensioactive agent |
Databáze: | OpenAIRE |
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