Comparison of eight methods to isolate genomic DNA from Hancornia speciosa
| Autor: | Marcones Ferreira Costa, Sérgio Emílio dos Santos Valente, P.P. Martins, V.M. Almeida, Paulo Sarmanho da Costa Lima, G. A. Luz, Maria Fernanda da Costa Gomes |
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| Přispěvatelé: | UFPI, PAULO SARMANHO DA COSTA LIMA, CPAMN, UFPI. |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA isolation Genetic diversity Reação em cadeia da polimerase Isolamento do DNA General Medicine Biology DNA extraction law.invention 03 medical and health sciences genomic DNA chemistry.chemical_compound 030104 developmental biology chemistry Mangabeira law Botany Agarose gel electrophoresis Genetics polymerase chain reaction Genetic erosion Molecular Biology Polymerase chain reaction DNA Fruit tree |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr16039724 |
| Popis: | Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits. |
| Databáze: | OpenAIRE |
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