Low cerebral blood flow is a non-invasive biomarker of neuroinflammation after repetitive mild traumatic brain injury
| Autor: | Levi B. Wood, Erin M. Buckley, Bharat Sanders, Kajol J. Shah, Sitara B. Sankar, Amanda Liew, Alyssa F. Pybus |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Traumatic brain injury Article lcsh:RC321-571 03 medical and health sciences Mice 0302 clinical medicine Western blot Neuroinflammation Kinase signaling Medicine Animals Mild traumatic brain injury Protein kinase B lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry PI3K/AKT/mTOR pathway Brain Concussion Inflammation medicine.diagnostic_test business.industry Brain Cerebral blood flow medicine.disease Mice Inbred C57BL 030104 developmental biology Neurology Cerebrovascular Circulation Biomarker (medicine) Immunohistochemistry Cytokines business 030217 neurology & neurosurgery |
| Zdroj: | Neurobiology of Disease, Vol 124, Iss, Pp 544-554 (2019) |
| Popis: | Previous work has shown that non-invasive optical measurement of low cerebral blood flow (CBF) is an acute biomarker of poor long-term cognitive outcome after repetitive mild traumatic brain injury (rmTBI). Herein, we explore the relationship between acute cerebral blood flow and underlying neuroinflammation. Specifically, because neuroinflammation is a driver of secondary injury after TBI, we hypothesized that both glial activation and inflammatory signaling are associated with acute CBF and, by extension, with long-term cognitive outcome after rmTBI. To test this hypothesis, cortical CBF was non-invasively measured in anesthetized mice 4 h after 3 repetitive closed head injuries spaced once-daily, at which time brains were collected. Right hemispheres were fixed for immunohistochemical staining for glial activation markers Iba1 and GFAP while left hemispheres were used to quantify Iba1 and GFAP expression via Western blot as well as 32 cytokines and 21 phospho-proteins in the MAPK, PI3K/Akt, and NF-κB pathways using a Luminex multiplexed immunoassay. N = 8/7 injured/sham C57/black-6 adult male mice were studied. Within the injured group, CBF inversely correlated with Iba1 expression (R = −0.86, p |
| Databáze: | OpenAIRE |
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