The Influence of Parathyroid Hormone and Its Fragments on Results from Midregion and C-Terminal Specific Radioimmunoassays*)
| Autor: | Matthias Rothmund, Krause U, Wagner Pk, J. Beyer, N. Kübler |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty Adolescent endocrine system diseases Endocrinology Diabetes and Metabolism Radioimmunoassay Parathyroid hormone Biology complex mixtures fluids and secretions Endocrinology Reference Values Internal medicine Internal Medicine medicine Humans Child Normal range Aged Aged 80 and over Hyperparathyroidism General Medicine Middle Aged Intact pth medicine.disease Peptide Fragments Specific radioimmunoassay Parathyroid Hormone Child Preschool Female Hyperparathyroidism Secondary hormones hormone substitutes and hormone antagonists Primary hyperparathyroidism Hormone |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 89:61-69 |
| ISSN: | 1439-3646 0947-7349 |
| Popis: | We compared a midregion (44-68) human parathyroid hormone (hPTH) specific radioimmunoassay (M-RIA) with a C-terminal (65-84) hPTH specific radioimmunoassay (C-RIA). The M-RIA discriminated 21 of 23 patients with primary hyperparathyroidism from 95 normals (normal range: 22.4-106.5 pmol/l). With the C-RIA 12 patients including the 2 patients not discriminated by the M-RIA had immunoreactive PTH (iPTH) values within the normal range of this assay (normal range: undetectable to 88.6 pmol/l). To investigate the reasons for these different abilities of separation, hyperparathyroid sera were subjected to gel-filtration and analyzed using the two assays. Intact PTH appeared to have a major influence on the immunoreactivity of circulating PTH in the M-RIA. In contrast, the C-RIA showed the highest immunoreactivity with midregion-C-terminal PTH fragments. Hyperparathyroid sera not discriminated by the C-RIA but with elevated iPTH in the M-RIA showed decreased amounts of midregion-C-terminal PTH fragments, while intact PTH comprised the highest amount of total circulating PTH immunoreactivity in the M-RIA. From the present results we conclude that the superiority of the M-RIA is due to the determination of intact PTH which is preferable for clinical measurements with relation to the diagnosis of primary hyperparathyroidism. |
| Databáze: | OpenAIRE |
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