Valproic acid-induced gene expression responses in rat whole embryo culture and comparison across in vitro developmental and non-developmental models
| Autor: | Joshua F. Robinson, Aart Verhoef, Jeroen L. A. Pennings, Elisa C.M. Tonk, Aldert H. Piersma, Peter T. Theunissen |
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| Přispěvatelé: | Toxicogenomics, Promovendi ODB, RS: GROW - School for Oncology and Reproduction |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Embryo
Nonmammalian Toxicology Transcriptome In vitro Gene expression Valproic acid Animals Rats Wistar Transcriptomics Zebrafish Embryonic Stem Cells Oligonucleotide Array Sequence Analysis Regulation of gene expression Genetics biology Gene Expression Profiling Gene Expression Regulation Developmental Embryo Embryo culture biology.organism_classification Embryo Mammalian Embryonic stem cell Cell biology Rats Teratogens Whole embryo culture lipids (amino acids peptides and proteins) |
| Zdroj: | Reproductive Toxicology, 41, 57-66. Elsevier Inc. |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2013.06.069 |
| Popis: | Transcriptomic evaluations may improve toxicity prediction of in vitro-based developmental models. In this study, transcriptomics was used to identify VPA-induced gene expression changes in rat whole embryo culture (WEC). Furthermore, VPA-induced responses were compared across in vitro-based developmental models, such as the cardiac and neural embryonic stem cells (ESTc and ESTn, respectively) and the zebrafish embryotoxicity model. VPA-induced gene regulation in WEC corresponded with observed morphological effects and previously suggested mechanisms of toxicity. Gene Ontology term-directed analysis showed conservation of VPA-induced gene expression changes across in vitro-based developmental models, with ESTc and ESTn exhibiting complementary responses. Furthermore, comparison of in vitro-based developmental and non-developmental models revealed that more generalized VPA-induced effects can be detected using non-developmental models whereas developmental models provide added value when assessing developmental-specific effects. These analyses can be used to optimize test batteries for the detection of developmental toxicants in vitro. |
| Databáze: | OpenAIRE |
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