Expression of the core promoter factors TATA box binding protein and TATA box binding protein-related factor 2 in Drosophila germ cells and their distinct functions in germline development
| Autor: | Masanori Mukai, Akane Kondo, Seiji Hira, Makoto Kojima, Shoichi Nakamura |
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| Rok vydání: | 2019 |
| Předmět: |
Biology
Germline 03 medical and health sciences 0302 clinical medicine Transcriptional regulation Animals Drosophila Proteins RNA Messenger Promoter Regions Genetic Gene 030304 developmental biology 0303 health sciences Gene knockdown TATA-Binding Protein Associated Factors General transcription factor TATA-Box Binding Protein RNA Promoter Cell Biology Cell biology Germ Cells Drosophila Transcription Factor TFIID 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development, growthdifferentiationREFERENCES. 62(9) |
| ISSN: | 1440-169X |
| Popis: | In Drosophila, the expression of germline genes is initiated in primordial germ cells (PGCs) and its expression is known to be associated with germline establishment. However, the transcriptional regulation of germline genes remains elusive. Previously, we found that the BTB/POZ-Zn-finger protein, Mamo, is necessary for the expression of the germline gene, vasa, in PGCs. Moreover, the truncated Mamo lacking the BTB/POZ domain (MamoAF) is a potent vasa activator. In this study, we investigated the genetic interaction between MamoAF and specific transcriptional regulators to gain insight into the transcriptional regulation of germline development. We identified a general transcription factor, TATA box binding protein (TBP)-associated factor 3 (TAF3/BIP2), and a member of the TBP-like proteins, TBP-related factor 2 (TRF2), as new genetic modifiers of MamoAF. In contrast to TRF2, TBP was found to show no genetic interaction with MamoAF, suggesting that Trf2 has a selective function. Therefore, we focused on Trf2 expression and investigated its function in germ cells. We found that the Trf2 mRNA, rather than the Tbp mRNA, was preferentially expressed in PGCs during embryogenesis. The depletion of TRF2 in PGCs resulted in decreased mRNA expression of vasa. RNA interference-mediated knockdown showed that while Trf2 is required for the maintenance of germ cells, Tbp is needed for their differentiation during oogenesis. Therefore, these results suggest that Trf2 and Tbp expression is differentially regulated in germ cells, and that these factors have distinct functions in Drosophila germline development. |
| Databáze: | OpenAIRE |
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