Absence of herpesvirus DNA by polymerase chain reaction in ocular fluids obtained from immunocompetent patients
| Autor: | Scott D. Pendergast, Danny L. Wiedbrauk, Ann M. Drevon, Jane C. Werner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Adolescent Hepatitis B virus DNA polymerase viruses Enzyme-Linked Immunosorbent Assay medicine.disease_cause Antibodies Viral Polymerase Chain Reaction Virus law.invention Aqueous Humor Immunocompromised Host law medicine Humans Child Polymerase chain reaction Herpesviridae Aged Aged 80 and over business.industry Varicella zoster virus virus diseases Infant Cytomegalovirus Retinal Necrosis Syndrome Acute General Medicine Middle Aged medicine.disease Virology Vitreous Body Ophthalmology Herpes simplex virus Immunoglobulin M Child Preschool Immunoglobulin G Cytomegalovirus Retinitis DNA Viral Female Cytomegalovirus retinitis Acute retinal necrosis business |
| Zdroj: | Retina (Philadelphia, Pa.). 20(4) |
| ISSN: | 0275-004X |
| Popis: | Objective To assess the prevalence of herpesvirus DNA in ocular fluids obtained from healthy patients undergoing vitreoretinal surgery. Background Polymerase chain reaction (PCR) has been used to detect herpesvirus DNA in patients with acute retinal necrosis and cytomegalovirus retinitis. Little is known regarding the prevalence of detectable herpesvirus DNA in ocular fluids collected from healthy seropositive patients with no clinical evidence of viral retinitis. Methods Seventy-five intraocular specimens (35 aqueous and 40 vitreous samples) were collected from 75 patients undergoing scleral buckling or vitrectomy. Using a PCR-based assay, the authors tested each specimen for the presence of herpesvirus genome DNA with primers specific for cytomegalovirus, Epstein-Barr virus, herpes simplex virus types 1 and 2, and varicella zoster virus. Serologic testing for immunoglobulin G (IgG) and IgM levels corresponding to each of the herpesviruses was also performed. Results Of the 75 samples tested, none was found to harbor herpesvirus DNA. The assay did not give false-positive results in patients with active intraocular inflammation. The sensitivity of the assay was 0.08 infection-forming units for cytomegalovirus, 0.6 tissue culture infectious doses for herpes simplex virus, 0.5 infected-cell equivalents for Epstein-Barr virus, and 0.03 focus-forming units for varicella zoster virus. The percentage of patients with positive herpesvirus serology ranged from 86% to 100% and was consistent with rates observed in the general population. Conclusions The prevalence of herpesvirus DNA detectable by PCR techniques in ocular fluids appears to be quite low despite the high proportion of patients who tested positive for herpesvirus antibodies. Therefore, a positive result obtained in a patient presenting with vitreoretinal inflammation should be regarded as significant. |
| Databáze: | OpenAIRE |
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