Bacillus amyloliquefaciens—Derived Lipopeptide Biosurfactants Inhibit Biofilm Formation and Expression of Biofilm-Related Genes of Staphylococcus aureus
| Autor: | Ján Király, Karolína Englerová, Rastislav Mucha, Zdenka Bedlovičová, Eva Styková, Radomíra Nemcová, Katarína Reiffová, Marián Maďar, Vanda Hajdučková |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Bacillus amyloliquefaciens Operon RM1-950 medicine.disease_cause Biochemistry Microbiology Article chemistry.chemical_compound medicine antibiofilm activity Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics biology Biofilm biosurfactant Lipopeptide Antimicrobial 16S ribosomal RNA biology.organism_classification lipopeptides Infectious Diseases chemistry Therapeutics. Pharmacology Surfactin |
| Zdroj: | Antibiotics, Vol 10, Iss 1252, p 1252 (2021) Antibiotics Volume 10 Issue 10 |
| ISSN: | 2079-6382 |
| Popis: | Biosurfactants (BSs) are surface-active compounds produced by diverse microorganisms, including the genus Bacillus. These bioactive compounds possess biological activities such as antiadhesive, antimicrobial and antibiofilm effects that can lead to important applications in combating many infections. Based on these findings, we decided to investigate the antibiofilm activity of BSs from the marine Bacillus amyloliquefaciens against Staphylococcus aureus CCM 4223. Expression of biofilm-related genes was also evaluated using qRT-PCR. Isolated and partially purified BSs were identified and characterized by molecular tools and by UHPLC-DAD and MALDI-TOF/MS. Bacillus amyloliquefaciens 3/22, that exhibited surfactant activity evaluated by oil spreading assay, was characterized using the 16S rRNA sequencing method. Screening by PCR detected the presence of the sfp, srfAA, fenD and ituD genes, suggesting production of the lipopeptides (LPs) surfactin, fengycin and iturin. The above findings were further supported by the results of UHPLC-DAD and MALDI-TOF/MS. As quantified by the crystal violet method, the LPs significantly (p < 0.001) reduced biofilm formation of S. aureus in a dose-dependent manner and decreased expression of biofilm-related genes fnbA, fnbB, sortaseA and icaADBC operon. Data from our investigation indicate a promising therapeutic application for LPs isolated from B. amyloliquefaciens toward prevention of S. aureus biofilm infections. |
| Databáze: | OpenAIRE |
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