Biochemical Maturation of Fetal Rat Lung: A Comprehensive Study Including Surfactant Determination
| Autor: | Cécile Valenza, Philip M. Farrell, Jeannette Brown, Edith Doucet, Jacques R. Bourbon |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
medicine.medical_specialty Gestational Age Biology Andrology chemistry.chemical_compound Pulmonary surfactant Internal medicine Phosphatidylcholine medicine Animals Lung Phospholipids Sex Characteristics Fetus Pulmonary Surfactants Rats Inbred Strains Rats Endocrinology medicine.anatomical_structure Animals Newborn chemistry Pediatrics Perinatology and Child Health Gestation Female Fetal lung Whole body Developmental Biology |
| Zdroj: | Neonatology. 52:48-60 |
| ISSN: | 1661-7819 1661-7800 |
| DOI: | 10.1159/000242684 |
| Popis: | The paper describes a comprehensive assessment of lung growth and biochemical maturation in the fetal and early postnatal rat. Fetal lung grew faster than whole body between gestational day 16.5 and term. Cell number increased quasi-exponentially, except for a slowing of cell growth between days 19.5 and 20.5 of gestation. By contrast, growth was limited during the two first postnatal days. Percentage of dry tissue and protein concentration increased in parallel during the whole period. Increases in whole lung tissue phosphatidylcholine were greatest between 20.5 and 21.5 days of gestation, whereas in isolated surfactant fraction, changes were most marked between 19.5 and 20.5 days. The most striking changes were a 19-fold increase in phosphatidylcholine and a 12.5-fold increase in the total phospholipid concentrations in the surfactant fraction between gestational day 19.5 and 1 day postpartum. Similar changes occurred in disaturated phosphatidylcholine (DSPC) concentration. During the same period, phospholipids of the residual (nonsurfactant) fraction increased only about 2-fold. These data indicate that analysis of an isolated surfactant fraction is advantageous in providing a very sensitive index of augmented phospholipid production during the process of fetal lung maturation. Evidence of biochemical maturation was detected earlier in females than in males, as indicated by a significantly larger surfactant fraction DSPC concentration in 19.5-day-old females; however, this difference was modest in degree and very transient, since it was no longer demonstrable in later stages. |
| Databáze: | OpenAIRE |
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