Regulation by protein kinase of phagocytosis of Mycobacterium leprae by macrophages
| Autor: | Harris Eb, B. Randhawa, K Prabhakaran |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
Phagocytosis Genistein Microbiology Pathogenesis Mice chemistry.chemical_compound medicine Animals Macrophage Staurosporine Enzyme Inhibitors Protein kinase A Protein Kinase Inhibitors Mycobacterium leprae Cells Cultured Mice Inbred BALB C biology General Medicine biology.organism_classification Hydroquinones Mice Inbred C57BL chemistry Herbimycin Macrophages Peritoneal Protein Kinases medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 49:339-342 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/0022-1317-49-4-339 |
| Popis: | Mycobacterium leprae multiplies within host macrophages. The mechanism of internalisation of the bacteria by the phagocytic cells is unknown. In this study, M. leprae was purified from the foot pads of experimentally infected nu/nu mice. Peritoneal macrophages were harvested from BALB/c mice or C57 beige (bg/bg) mice. The effect of protein kinase inhibitors (erbstatin, genistein or staurosporine for BALB/c and bg/bg mice, plus herbimycin for bg/bg mice) on phagocytosis of the mycobacteria by the macrophage monolayers was tested. The untreated (control) macrophages phagocytosed M. leprae. Phagocytosis by BALB/c macrophages was inhibited by erbstatin and staurosporine but not by genistein; all the protein kinase inhibitors prevented uptake of M. leprae by bg/bg cells. The results demonstrate that protein kinase regulates phagocytosis of M. leprae by macrophages. The mechanism might prove to be a rational drug target for mycobacteria that multiply intracellularly. |
| Databáze: | OpenAIRE |
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