Chitosan Nanoparticles as Carriers for the Delivery of ΦKAZ14 Bacteriophage for Oral Biological Control of Colibacillosis in Chickens
| Autor: | Adamu Ahmad Kaikabo, Farida Abas, Abdulkarim Sabo Mohammed |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
colibacillosis
0301 basic medicine chitosan nanoparticles 030106 microbiology Administration Oral Pharmaceutical Science Nanoparticle Coliphages Article Analytical Chemistry Microbiology lcsh:QD241-441 bacteriophage chickens Gastric Acid Chitosan Bacteriophage 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems Differential scanning calorimetry lcsh:Organic chemistry Drug Discovery Animals Particle Size Physical and Theoretical Chemistry Escherichia coli Infections Poultry Diseases chemistry.chemical_classification Gel electrophoresis biology Organic Chemistry biology.organism_classification Chorioallantoic membrane 030104 developmental biology Enzyme chemistry Chemistry (miscellaneous) Nanoparticles Molecular Medicine Particle size Nuclear chemistry |
| Zdroj: | Molecules Molecules; Volume 21; Issue 3; Pages: 256 Molecules, Vol 21, Iss 3, p 256 (2016) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules21030256 |
| Popis: | The use of chitosan as a delivery carrier has attracted much attention in recent years. In this study, chitosan nanoparticles (CS-NP) and chitosan-ΦKAZ14 bacteriophage-loaded nanoparticles (C-ΦKAZ14 NP) were prepared by a simple coercavation method and characterized. The objective was to achieve an effective protection of bacteriophage from gastric acids and enzymes in the chicken gastrointestinal tract. The average particle sizes for CS-NP and C-ΦKAZ14 NP were 188 ± 7.4 and 176 ± 3.2 nm, respectively. The zeta potentials for CS-NP and C-ΦKAZ14 NP were 50 and 60 mV, respectively. Differential scanning calorimetry (DSC) of C-ΦKAZ14 NP gave an onset temperature of −17.17 °C with a peak at 17.32 °C and final end set of 17.41 °C, while blank chitosan NP had an onset of −20.00 °C with a peak at −19.78 °C and final end set at −20.47. FT-IR spectroscopy data of both CS-NP and C-ΦKAZ14 NP were the same. Chitosan nanoparticles showed considerable protection of ΦKAZ14 bacteriophage against degradation by enzymes as evidenced in gel electrophoresis, whereby ΦKAZ14 bacteriophage encapsulated in chitosan nanoparticles were protected whereas the naked ΦKAZ14 bacteriophage were degraded. C-ΦKAZ14 NP was non-toxic as shown by a chorioallantoic membrane (CAM) toxicity assay. It was concluded that chitosan nanoparticles could be a potent carrier of ΦKAZ14 bacteriophage for oral therapy against colibacillosis in poultry. |
| Databáze: | OpenAIRE |
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