Bovine Staphylococcus aureus: Dose response to iodine and chlorhexidine and effect of iodine challenge on antibiotic susceptibility
| Autor: | Kevin L. Anderson, Roberta L. Lyman, Reha Onur Azizoglu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Staphylococcus aureus
medicine.drug_class Antibiotics chemistry.chemical_element Microbial Sensitivity Tests Drug resistance Iodine medicine.disease_cause Microbiology Mammary Glands Animal Drug Resistance Bacterial Genotype Genetics medicine Animals Mastitis Bovine Dose-Response Relationship Drug business.industry Chlorhexidine Staphylococcal Infections medicine.disease Mastitis Dose–response relationship chemistry Anti-Infective Agents Local Cattle Female Animal Science and Zoology business Food Science medicine.drug |
| Zdroj: | Journal of Dairy Science. 96:993-999 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2012-5857 |
| Popis: | Staphylococcus aureus is a gram-positive organism that is frequently associated with clinical or subclinical mastitis. The use of germicidal teat dips is one of the measures taken by the dairy industry to control mastitis. Iodine and chlorhexidine compounds are commonly used disinfectants in teat dips. We determined the minimum inhibitory concentrations (MIC) of iodine for 37 isolates of Staph. aureus and observed variations in MIC. Seven of these Staph. aureus isolates were selected as genotype group representatives based on their pulsed-field gel electrophoresis patterns. Dose responses against iodine and chlorhexidine were determined for the 7 genotype group representatives. The response of these isolates to iodine varied significantly, whereas all isolates were susceptible to chlorhexidine, even at concentrations as low as 0.0002%. We also evaluated whether exposure of Staph. aureus to sublethal levels of iodine influenced subsequent antibiotic susceptibility. No differences in antibiotic susceptibility of Staph. aureus were observed among cultures grown in brain heart infusion broth with and without supplemental iodine. The observed variation in iodine dose responses of Staph. aureus may have implications for the occurrence of Staph. aureus mastitis on dairy farms. |
| Databáze: | OpenAIRE |
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