The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay
| Autor: | Craig A. Smibert, Laura M. Rendl, Heli K. Vari, Melissa A. Bieman |
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| Rok vydání: | 2012 |
| Předmět: |
RNA Caps
RNA Stability Saccharomyces cerevisiae Proteins Eukaryotic Initiation Factor-4E lcsh:Medicine Gene Expression RNA-binding protein Yeast and Fungal Models Plasma protein binding Biology Biochemistry Molecular Genetics Model Organisms Molecular cell biology Nucleic Acids Genetics RNA Messenger lcsh:Science Protein Interactions Transcription factor Multidisciplinary EIF4E lcsh:R RNA RNA-Binding Proteins Proteins Molecular biology Cell biology RNA processing lcsh:Q Smaug Protein Binding Transcription Factors Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 10, p e47121 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Sequence-specific RNA binding proteins can induce the degradation of mRNAs through their ability to recruit proteins that trigger transcript destabilization. For example, Vts1p, the S. cerevisiae member of the Smaug family of RNA binding proteins, is thought to induce transcript decay by recruiting the Ccr4p-Pop2p-Not deadenylase complex to target mRNAs. The resulting deadenylation triggers transcript decapping followed by 5'-to-3' exonucleolytic decay. Here we show that the eIF4E-binding protein, Eap1p, is required for efficient degradation of Vts1p target transcripts and that this role involves the ability of Eap1p to interact with eIF4E. Eap1p does not stimulate deadenylation of Vts1p target transcripts but is instead involved in decapping. Eap1p interacts with Vts1p and mediates an indirect interaction between Vts1p and eIF4E. Taken together these data suggest a model whereby the interaction of Vts1p with Eap1p at target mRNAs stimulates decapping. |
| Databáze: | OpenAIRE |
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