Proteomics, Genomics, and Pathway Analyses of Escherichia coli and Staphylococcus aureus Infected Milk Whey Reveal Molecular Pathways and Networks Involved in Mastitis
| Autor: | Aloysius E Ibeagha, Serge Messier, Eveline M. Ibeagha-Awemu, Xin Zhao |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Proteomics
Staphylococcus aureus Biology medicine.disease_cause Biochemistry Microbiology Immune system Tandem Mass Spectrometry medicine Animals Mastitis Bovine Gene Escherichia coli Escherichia coli Infections Cell Line Transformed Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Genomics General Chemistry Staphylococcal Infections Milk Proteins medicine.disease biology.organism_classification Mastitis Milk Whey Proteins Real-time polymerase chain reaction Gene Expression Regulation Cattle Electrophoresis Polyacrylamide Gel Female Metabolic Networks and Pathways Bacteria Chromatography Liquid Signal Transduction |
| Zdroj: | Journal of Proteome Research. 9:4604-4619 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr100336e |
| Popis: | Gram-negative and -positive bacteria elicit different response patterns by the host. The proteomic profiles of milk whey samples from cows naturally infected with Escherichia coli or Staphyloccocus aureus as compared to whey from healthy cows were determined by one-dimensional, liquid chromatography-tandem mass spectrometry (LC-MS/MS), bioinformatics processing, and pathway analyses. Since mammary epithelial cells contribute to immune responses in mammary glands, the genes of selected proteins were measured in MAC-T cells by real time quantitative PCR (qPCR) after stimulation with heat inactivated E. coli strain P4 and S. aureus strain Smith CP bacteria. A total of 173 proteins were identified including 73 proteins differentially expressed among normal, E. coli, and S. aureus treatment groups. E. coli was more effective at significantly altering the concentration of the affected proteins. The mRNA of 23 proteins out of 24 measured by qPCR was significantly altered in MAC-T cells. Pathway analyses identified top canonical pathways significantly enriched in our samples, the most significant being the acute phase response signaling pathway. Also, top networks of genes with significant associations to identified proteins were identified. Our study has demonstrated a wider proteome profile of E. coli and S. aureus mastitic milk whey, identified more low abundant defense proteins than reported before, and has linked for the first time identified proteins to several network functions and Biocarta pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|