Species richness, distribution and genetic diversity of Caenorhabditis nematodes in a remote tropical rainforest

Autor: Christian Braendle, Shery Han, Erik C. Andersen, Céline Ferrari, Asher D. Cutter, Marie-Anne Félix, Young Ran Cho, Richard Jovelin
Přispěvatelé: Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Ecology and Evolutionary Biology, University of Toronto, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Princeton University -Lewis-Sigler Institute for Integrative Genomics, MAF and CB acknowledge financial support by the CNRS, including a grant for research at the Nouragues field station, and would like to thank the staff at the station and the CNRS in French Guiana, in particular, Patrick Châtelet, Philippe Gaucher and Jérôme Chave. We also thank those who provided access to sampling sites (Bruno Le Vessier) and information on sampled fruits (Pierre-Michel Forget). We are grateful to Matt Rockman for the New Jersey isolates and to Patrick Châtelet, Mathieu Joron, Didier Peigne, Christopher Nelson, Amir Yassin for collecting samples. CB is funded by the CNRS, Agence Nationale de la Recherche and the Fondation Schlumberger pour l'Education et la Recherche. ADC received support from the Natural Sciences and Engineering Research Council (NSERC) of Canada and from a Canada Research Chair. RJ was supported by an Ontario Ministry of Research and Innovation postdoctoral fellowship and SH was supported by a University of Toronto Excellence Award. ECA was supported by a Ruth L. Kirschstein National Research Service Award post-doctoral fellowship (F32-GM089007) and training grant T32-CA009528 from the National Cancer Institute., BMC, Ed., Institut de biologie de l'ENS Paris (IBENS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2013
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences
Evolution
Population
Biodiversity
Helminth genetics
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
C. briggsae
Population structure
010603 evolutionary biology
01 natural sciences
Trees
Nucleotide diversity
03 medical and health sciences
QH359-425
Animals
Caenorhabditis
education
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Ecosystem
Phylogeny
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Tropical Climate
[SDV.SA] Life Sciences [q-bio]/Agricultural sciences
0303 health sciences
Genetic diversity
education.field_of_study
biology
Ecology
Genetic Variation
Sequence Analysis
DNA
DNA
Helminth
15. Life on land
biology.organism_classification
French Guiana
[SDE.BE] Environmental Sciences/Biodiversity and Ecology
Fruit
Species richness
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Research Article
Tropical rainforest
Zdroj: BMC Evolutionary Biology
BMC Evolutionary Biology, BioMed Central, 2013, 13 (1), pp.10. ⟨10.1186/1471-2148-13-10⟩
BMC Evolutionary Biology, 2013, 13 (1), pp.10. ⟨10.1186/1471-2148-13-10⟩
BMC Evolutionary Biology, Vol 13, Iss 1, p 10 (2013)
ISSN: 1471-2148
DOI: 10.1186/1471-2148-13-10
Popis: Background In stark contrast to the wealth of detail about C. elegans developmental biology and molecular genetics, biologists lack basic data for understanding the abundance and distribution of Caenorhabditis species in natural areas that are unperturbed by human influence. Methods Here we report the analysis of dense sampling from a small, remote site in the Amazonian rain forest of the Nouragues Natural Reserve in French Guiana. Results Sampling of rotting fruits and flowers revealed proliferating populations of Caenorhabditis, with up to three different species co-occurring within a single substrate sample, indicating remarkable overlap of local microhabitats. We isolated six species, representing the highest local species richness for Caenorhabditis encountered to date, including both tropically cosmopolitan and geographically restricted species not previously isolated elsewhere. We also documented the structure of within-species molecular diversity at multiple spatial scales, focusing on 57 C. briggsae isolates from French Guiana. Two distinct genetic subgroups co-occur even within a single fruit. However, the structure of C. briggsae population genetic diversity in French Guiana does not result from strong local patterning but instead presents a microcosm of global patterns of differentiation. We further integrate our observations with new data from nearly 50 additional recently collected C. briggsae isolates from both tropical and temperate regions of the world to re-evaluate local and global patterns of intraspecific diversity, providing the most comprehensive analysis to date for C. briggsae population structure across multiple spatial scales. Conclusions The abundance and species richness of Caenorhabditis nematodes is high in a Neotropical rainforest habitat that is subject to minimal human interference. Microhabitat preferences overlap for different local species, although global distributions include both cosmopolitan and geographically restricted groups. Local samples for the cosmopolitan C. briggsae mirror its pan-tropical patterns of intraspecific polymorphism. It remains an important challenge to decipher what drives Caenorhabditis distributions and diversity within and between species.
Databáze: OpenAIRE
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