Protective Effects of Colchicine in an Experimental Rat Endometriosis Model: Histopathological Evaluation and Assessment of TNF-α Levels
| Autor: | Atilla Karateke, Dilek Benk Silfeler, Ali Ulvi Hakverdi, Raziye Keskin Kurt, Neslihan Pınar, Aynur Albayrak, Ayşe Güler Okyay, Seyda Özdemir |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors medicine.medical_treatment Anti-Inflammatory Agents Endometriosis Urology Down-Regulation Inflammation Endometrium chemistry.chemical_compound medicine Animals Ascitic Fluid Colchicine Rats Wistar Saline Cell Proliferation Tumor Necrosis Factor-alpha business.industry Peritoneal fluid Obstetrics and Gynecology medicine.disease Treatment period Disease Models Animal chemistry Cytoprotection Female Tumor necrosis factor alpha Implant medicine.symptom business |
| Zdroj: | Reproductive Sciences. 22:258-263 |
| ISSN: | 1933-7205 1933-7191 |
| DOI: | 10.1177/1933719114542029 |
| Popis: | Objective: Endometriosis is an estrogen-dependent chronic inflammatory disease observed in reproductive period. The aim of the present study is to assess the efficacy of colchicine, widely used to treat many inflammatory diseases, in an experimental rat endometriosis model. Study Design: Experimental endometriosis was constituted with implantation of autogenous endometrial tissue. Rats were divided randomly into 2 groups as colchicine group (n = 8) and control group (n =8). Although oral 0.1 mg/kg colchicine was administered 4 weeks to the colchicine group, the same amount of saline solution was administered to the control group. Before and after 30 days of treatment period, peritoneal and tissue tumor necrosis factor α (TNF-α), the volumes and histopathological properties of the implants were evaluated. Results: Although the implant volume decreased significantly in the colchicine group (89.2 ± 13.4 mm3 to 35.2 ± 4.5 mm3, P < .05), the implant volume increased in the control group (85.1 ± 14.2 mm3 to 110.3 ± 10.5 mm3, P < .05). When compared to the control group, the colchicine group had significantly lower histopathologic sores (1.4 ± 0.2 vs 2.6 ± 0.4, P < .001). Although peritoneal fluid TNF-α levels were significantly decreased in the colchicine group (45.2 ± 5.3 pg/mL vs 12.1 ± 5.2 pg/mL, P < .001), the peritoneal fluid TNF-α levels were significantly increased in the control group after the treatment (44.2 ± 3.5 pg/mL vs 61.3 ± 12.2 pg/mL; P < .001). Tissue TNF-α levels were significantly lower in the colchicine group when compared to the control group (45.4 ± 8.6 pg/mL vs 71.3 ± 11.2 pg/mL; P < .001). Conclusion: Colchicine resulted in regression of endometrial implant volumes in experimental rat endometriosis model and decreased peritoneal and tissue TNF-α levels. |
| Databáze: | OpenAIRE |
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