Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies
| Autor: | Davide Porcellato, Morten Tryland, Jane Ludvigsen, Jannice Schau Slettemeås, Anuradha Ravi, Amar A. Telke, Erik Ropstad, Knut Rudi, Carlos G. das Neves, Marianne Sunde, Silje Nøstvedt Ramstad |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Immunology Virulence medicine.disease_cause Antimicrobial resistance Microbiology Svalbard Plasmid Antibiotic resistance biology.animal Drug Resistance Bacterial medicine VDP::Mathematics and natural science: 400::Zoology and botany: 480 Escherichia coli Immunology and Allergy Animals Arctic fox Ecosystem IncF plasmid biology Svalbard reindeer biology.organism_classification QR1-502 Anti-Bacterial Agents Wild reindeer Colicin Bacteria Plasmids Reindeer VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 |
| Zdroj: | Journal of Global Antimicrobial Resistance Journal of Global Antimicrobial Resistance, Vol 26, Iss, Pp 317-322 (2021) |
| Popis: | Objectives - In extreme environments, such as the Arctic region, the anthropogenic influence is low and the presence of antimicrobial-resistant bacteria is unexpected. In this study, we screened wild reindeer (Rangifer tarandus platyrhynchus) from the Svalbard High Arctic Archipelago for antimicrobial-resistant Escherichia coli and performed in-depth strain characterisation. Methods - Using selective culturing of faecal samples from 55 animals, resistant E. coli were isolated and subjected to minimum inhibitory concentration (MIC) determination, conjugation experiments and whole-genome sequencing. Results - Twelve animals carried antimicrobial-resistant E. coli. Genomic analysis showed IncF plasmids as vectors both for resistance and virulence genes in most strains. Plasmid-associated genes encoding resistance to ampicillin, sulfonamides, streptomycin and trimethoprim were found in addition to virulence genes typical for colicin V (ColV)-producing plasmids. Comparison with previously reported IncF ColV plasmids from human and animal hosts showed high genetic similarity. The plasmids were detected in E. coli sequence types (STs) previously described as hosts for such plasmids, such as ST58, ST88 and ST131. Conclusion - Antimicrobial-resistant E. coli were detected from Svalbard reindeer. Our findings show that successful hybrid antimicrobial resistance–ColV plasmids and their host strains are widely distributed also occurring in extreme environmental niches such as arctic ecosystems. Possible introduction routes of resistant bacterial strains and plasmids into Svalbard ecosystems may be through migrating birds, marine fish or mammals, arctic fox (Vulpes lagopus) or via human anthropogenic activities such as tourism. |
| Databáze: | OpenAIRE |
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