Magnetic mapping of iron in rodent spleen
| Autor: | Gunjan Agarwal, Brittany M. Penn, Brooke Ollander, Angela R. Blissett, Dana M. McTigue |
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| Rok vydání: | 2016 |
| Předmět: |
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Materials science Iron Biomedical Engineering Analytical chemistry Pharmaceutical Science Medicine (miscellaneous) Bioengineering Spleen 02 engineering and technology 010402 general chemistry Microscopy Atomic Force 01 natural sciences Article Rats Sprague-Dawley Magnetics medicine Animals General Materials Science biology Staining and Labeling Resolution (electron density) Histology Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences Staining Ferritin medicine.anatomical_structure Transmission electron microscopy Biophysics biology.protein Ultrastructure Molecular Medicine Magnetic force microscope 0210 nano-technology |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 13(3) |
| ISSN: | 1549-9642 |
| Popis: | Evaluation of iron distribution and density in biological tissues is important to understand the pathogenesis of a variety of diseases and the fate of exogenously administered iron-based carriers and contrast agents. Iron distribution in tissues is typically characterized via histochemical (Perl's) stains or immunohistochemistry for ferritin, the major iron storage protein. A more accurate mapping of iron can be achieved via ultrastructural transmission electron microscopy (TEM) based techniques, which involve stringent sample preparation conditions. In this study, we elucidate the capability of magnetic force microscopy (MFM) as a label-free technique to map iron at the nanoscale level in rodent spleen tissue. We complemented and compared our MFM results with those obtained using Perl's staining and TEM. Our results show how MFM mapping corresponded to sizes of iron-rich lysosomes at a resolution comparable to that of TEM. In addition MFM is compatible with tissue sections commonly prepared for routine histology. |
| Databáze: | OpenAIRE |
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