Association betweenFaecalibacterium prausnitziiand dietary fibre in colonic fermentation in healthy human subjects
| Autor: | Gjalt W. Welling, Robin F. J. Benus, Moira A. Taylor, Hermie J. M. Harmsen, Tjip S. van der Werf, Patricia A. Judd, Kevin Whelan |
|---|---|
| Přispěvatelé: | Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI) |
| Rok vydání: | 2010 |
| Předmět: |
Adult
Dietary Fiber Male Colon BUTYRATE-PRODUCING BACTERIA Medicine (miscellaneous) Faecalibacterium prausnitzii Butyrate Microbiology Feces Young Adult FUSOBACTERIUM-PRAUSNITZII Humans Food science Human feces TARGETED OLIGONUCLEOTIDE PROBES Nutrition and Dietetics Bacteria biology RNA-BASED PROBES Microbiota Dietary fibre IN-SITU HYBRIDIZATION HUMAN INTESTINAL MICROBIOTA Fusobacterium Fatty Acids Volatile biology.organism_classification CROHNS-DISEASE Diet Anaerobic bacteria Butyrates CHAIN FATTY-ACIDS Butyrate-Producing Bacteria Fermentation FECAL MICROBIOTA Female Roseburia Energy source HUMAN FECES Molecular diagnostic techniques |
| Zdroj: | British Journal of Nutrition, 104(5), 693-700. Cambridge University Press |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s0007114510001030 |
| Popis: | The intestinal microbiota are a complex ecosystem influencing the immunoregulation of the human host, providing protection from colonising pathogens and producing SCFA as the main energy source of colonocytes. Our objective was to investigate the effect of dietary fibre exclusion and supplementation on the intestinal microbiota and SCFA concentrations. Faecal samples were obtained from healthy volunteers before and after two 14 d periods of consuming formulated diets devoid or supplemented with fibre (14 g/l). The faecal microbiota were analysed using fluorescentin situhybridisation and SCFA were measured using GLC. There were large and statistically significant reductions in the numbers of theFaecalibacterium prausnitzii(P ≤ 0·01) andRoseburiaspp. (P ≤ 0·01) groups during both the fibre-free and fibre-supplemented diets. Significant and strong positive correlations between the proportion ofF. prausnitziiand the proportion of butyrate during both baseline normal diets were found (pre-fibre freer0·881,P = 0·001; pre-fibre supplementedr0·844,P = 0·002). A significant correlation was also found between the proportional reduction inF. prausnitziiand the proportional reduction in faecal butyrate during both the fibre-free (r0·806;P = 0·005) and the fibre-supplemented diet (r0·749;P = 0·013). These findings may contribute to the understanding of the association between fibre, microbiota and fermentation in health, during enteral nutrition and in disease states such as Crohn's disease. |
| Databáze: | OpenAIRE |
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