Variation in Colonization, ADP-Ribosylating and Vacuolating Cytotoxin, and Pulmonary Disease Severity among Mycoplasma pneumoniae Strains
| Autor: | Pooya Iranpour, Thirumalai R Kannan, Claudia Tagliabue, Marianna Cagle, Chonnamet Techasaensiri, Jacqueline J. Coalson, Joel B. Baseman, R. Doug Hardy, Kathy H. Katz |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lung Diseases Mycoplasma pneumoniae Bacterial Toxins Colony Count Microbial Mycoplasmataceae Critical Care and Intensive Care Medicine medicine.disease_cause Severity of Illness Index Microbiology Mice fluids and secretions Bacterial Proteins Intensive care Pneumonia Mycoplasma medicine Animals Mice Inbred BALB C medicine.diagnostic_test biology Toxin Cytotoxins Respiratory disease biology.organism_classification medicine.disease bacterial infections and mycoses digestive system diseases respiratory tract diseases E. Pulmonary Infections Pneumonia Disease Models Animal Bronchoalveolar lavage Mollicutes bacteria Female Bronchoalveolar Lavage Fluid |
| Popis: | Mycoplasma pneumoniae was recently discovered to produce an ADP-ribosylating and vacuolating cytotoxin, designated CARDS toxin, which is hypothesized to be a primary pathogenic mechanism responsible for M. pneumoniae-induced pulmonary inflammation. It is unknown if cytotoxin production varies with M. pneumoniae strain or if variation in cytotoxin production affects pulmonary disease severity.To examine the production of CARDS toxin by various strains of M. pneumoniae and compare the disease manifestations elicited by these strains in an experimental model of M. pneumoniae respiratory infection.BALB/c mice were inoculated once intranasally with SP4 broth (negative control) or three different M. pneumoniae strains: M129-B7, M129-B9, or S1. Mice were assessed at 1, 2, 4, 7, 10, and 14 days after inoculation. Outcome variables included comparisons among M. pneumoniae strains relative to bronchoalveolar lavage (BAL) M. pneumoniae quantitative culture, CARDS toxin-based PCR, and CARDS toxin protein determinations, as well as cytokine and chemokine concentrations. Graded lung histopathologic score (HPS) was also assessed.CARDS toxin concentrations were significantly increased in mice inoculated with strain S1 compared with mice inoculated with M129-B7 or M129-B9 strains. Quantitative M. pneumoniae culture and polymerase chain reaction were also significantly greater in mice infected with S1 strain compared with the other two strains, as were lung HPS and concentrations of IFN-γ, IL-12, IL-1α, macrophage inflammatory protein-1α, and keratinocyte-derived chemokine. In addition, a significant positive correlation was found between CARDS toxin concentration and lung HPS.CARDS toxin concentrations in BAL are directly linked to the ability of specific M. pneumoniae strains to colonize, replicate, and persist, and elicit lung histopathology. This variation among strains may predict the range in severity of pulmonary disease observed among patients. |
| Databáze: | OpenAIRE |
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