Transcript mapping of abutilon mosaic virus, A geminivirus
| Autor: | Thomas Frischmuth, Sabine Frischmuth, Holger Jeske |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Polyadenylation Molecular Sequence Data Genome Viral Abutilon mosaic virus Genome Primer extension chemistry.chemical_compound Mosaic Viruses Transcription (biology) Virology Complementary DNA Amino Acid Sequence Cloning Molecular Genetics Base Sequence biology Nucleic acid sequence Blotting Northern biology.organism_classification Molecular biology Oligodeoxyribonucleotides chemistry DNA Viral DNA Circular Poly A DNA |
| Zdroj: | Virology. 185:596-604 |
| ISSN: | 0042-6822 |
| Popis: | The transcripts of the DNA of Abutilon mosaic virus, a geminivirus with a bipartite genome (DNA A and DNA B), were characterized by Northern blot hybridization, S1 nuclease assay, primer extension analysis, and sequencing of the 3′ termini of cDNA clones. It was shown that transcription is bidirectional and that the transcripts are polyadenylated. Two overlapping transcripts of 1.6 and 0.7 kb were mapped to the complementary strand of DNA A and two of 1.3 and 1.2 kb to the complementary strand of DNA B. One transcript of 0.9 kb was mapped to the viral sense in DNA A and one of 1.0 kb to the viral sense in DNA B. The ends of complementary and viral mRNA overlapped in both genome parts in regions rich in polyadenylation signals. |
| Databáze: | OpenAIRE |
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