Bacillus pumilus 15.1, a Strain Active against Ceratitis capitata, Contains a Novel Phage and a Phage-Related Particle with Bacteriocin Activity

Autor:	Susana Vílchez, Alberto Fernández-Fernández, Antonio Osuna
Rok vydání:	2021
Předmět:	QH301-705.5 Virulence Factors viruses bacteriocin activity Virulence Entomopathogen Article Catalysis Microbiology Inorganic Chemistry Bacteriophage 03 medical and health sciences chemistry.chemical_compound bacteriophage Bacteriocins PBSX Animals Bacteriophages Bacillus pumilus Biology (General) Physical and Theoretical Chemistry ORFS QD1-999 Molecular Biology Spectroscopy 030304 developmental biology Bacillus (shape) 0303 health sciences biology Strain (chemistry) 030306 microbiology fungi Organic Chemistry Ceratitis capitata Bacteriocin activity General Medicine biology.organism_classification entomopathogen Computer Science Applications Chemistry chemistry DNA
Zdroj:	Digibug. Repositorio Institucional de la Universidad de Granada instname International Journal of Molecular Sciences, Vol 22, Iss 8164, p 8164 (2021) International Journal of Molecular Sciences Volume 22 Issue 15
ISSN:	1422-0067
Popis:	This research was partially funded by the Andalucian Operative Program, grant number B-BIO-081-UGR18. All authors have read and agreed to the published version of the manuscript. A 98.1 Kb genomic region from B. pumilus 15.1, a strain isolated as an entomopathogen toward C. capitata, the Mediterranean fruit fly, has been characterised in search of potential virulence factors. The 98.1 Kb region shows a high number of phage-related protein-coding ORFs. Two regions with different phylogenetic origins, one with 28.7 Kb in size, highly conserved in Bacillus strains, and one with 60.2 Kb in size, scarcely found in Bacillus genomes are differentiated. The content of each region is thoroughly characterised using comparative studies. This study demonstrates that these two regions are responsible for the production, after mitomycin induction, of a phage-like particle that packages DNA from the host bacterium and a novel phage for B. pumilus, respectively. Both the phage-like particles and the novel phage are observed and characterised by TEM, and some of their structural proteins are identified by protein fingerprinting. In addition, it is found that the phage-like particle shows bacteriocin activity toward other B. pumilus strains. The effect of the phage-like particles and the phage in the toxicity of the strain toward C. capitata is also evaluated. Andalucian Operative Program B-BIO-081-UGR18
Databáze:	OpenAIRE
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