Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study
| Autor: | Milena Krajnović, Nada Kraguljac-Kurtovic, Mirjana Gotic, Koviljka Krtolica, Bogomir Dimitrijević, Biljana Mihaljevic |
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| Rok vydání: | 2010 |
| Předmět: |
Methyltransferase
B-cell non-Hodgkin lymphoma O6-methylguanine-DNA methyltransferase Disease Biology Methylation status DNA methyltransferase General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine immune system diseases hemic and lymphatic diseases medicine lcsh:QH301-705.5 neoplasms Gene O-6-methylguanine-DNA methyltransferase 030304 developmental biology 0303 health sciences p15INK4B Methylation medicine.disease Molecular biology digestive system diseases 3. Good health Lymphoma lcsh:Biology (General) 030220 oncology & carcinogenesis DNA methylation B-Cell Non-Hodgkin Lymphoma Cancer research General Agricultural and Biological Sciences p15(INK4B) |
| Zdroj: | Archives of Biological Sciences, Vol 62, Iss 2, Pp 211-221 (2010) Archives of Biological Sciences |
| ISSN: | 1821-4339 0354-4664 |
| Popis: | The methylation status of the target promoter sequences of p15INK4B (p15) and O6-methylguanine-DNA methyltransferase (MGMT) genes was studied by methylation-specific PCR in 10 adult patients with de novo B-cell non- Hodgkin lymphoma (B-NHL). The aberrant hypermethylation of the p15 gene was more frequent (50%) compared to the hypermethylation of the MGMT gene (30%), and was detected in different types of B-NHL in both genes. Hypermethylation of the MGMT gene occurred exclusively in association with the hypermethylation of the p15 gene. All lymphoma patients with hypermethylation of the p15 and/or MGMT genes had a higher clinical stage of the disease (IV - V). We show the association of anemia and/or thrombocytopenia with the hypermethylation of the p15 gene, ascribing the p15 gene as a potential prognostic marker in B-NHL. Comethylation of MGMT with the p15 gene represents a novel finding and presents both genes as candidates for future studies of the hypermethylation profiles of B-NHL. |
| Databáze: | OpenAIRE |
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