E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow
| Autor: | Cecilia Grimaldi, Jean-Christophe Olivo-Marin, Jan Schick, Jan Bandemer, Erez Raz, Aleix Boquet-Pujadas, Isabel Schumacher, Timo Betz, Anne Aalto, Bart Vos, Katsiaryna Tarbashevich |
|---|---|
| Přispěvatelé: | Zentrum für Molekularbiologie der Entzündung - Center for Molecular Biology of Inflammation [Münster, Germany] (ZMBE), Westfälische Wilhelms-Universität Münster (WWU), Analyse d'images biologiques - Biological Image Analysis (BIA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), This work was supported by the European Research Council (ERC, CellMig), the German Research Foundation (DFG, CRC 1348 and RA 863/11-1), The Medical Faculty of the University of Muenster and the Cells in Motion cluster. C.G. is a member of the CiM-IMPRS Graduate School. A.B.-P. is part of the Pasteur-Paris University (PPU) International PhD Programme, which received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 665807, and from the Institut Carnot Pasteur Microbes & Santé (ANR-16 CARN 0023-01). The BIA unit is partially supported by grants from the Labex IBEID (ANR-10-LABX-62-IBEID), France-BioImaging infrastructure (ANR-10-INBS-04), and the programme PIA INCEPTION (ANR-16-CONV-0005). T.B. and B.E.V. were supported by the European Research Council (Consolidator Grant 771201). Open Access funding enabled and organized by Projekt DEAL, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016), European Project: 268806,EC:FP7:ERC,ERC-2010-AdG_20100317,CELLMIG(2011), European Project: 665807,H2020,H2020-MSCA-COFUND-2014,PASTEURDOC(2015), Westfälische Wilhelms-Universität Münster = University of Münster (WWU), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Embryology [SDV]Life Sciences [q-bio] Cell General Physics and Astronomy MESH: Actomyosin MESH: Cadherins 0302 clinical medicine Cell Movement Adherens junctions MESH: Animals Pseudopodia lcsh:Science MESH: Cell Movement Zebrafish Multidisciplinary biology Chemistry Actomyosin Adhesion Cadherins Cell biology medicine.anatomical_structure Female Leading edge Cell type Science MESH: Zebrafish Proteins [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Actins Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences medicine Animals Cell migration MESH: Zebrafish Actin Cadherin Front (oceanography) General Chemistry Zebrafish Proteins biology.organism_classification Actins MESH: Male MESH: Germ Cells Germ Cells 030104 developmental biology MESH: Pseudopodia lcsh:Q MESH: Female 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.5397. ⟨10.1038/s41467-020-19114-z⟩ Nature Communications, 2020, 11 (1), pp.5397. ⟨10.1038/s41467-020-19114-z⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-19114-z⟩ |
| Popis: | The migration of many cell types relies on the formation of actomyosin-dependent protrusions called blebs, but the mechanisms responsible for focusing this kind of protrusive activity to the cell front are largely unknown. Here, we employ zebrafish primordial germ cells (PGCs) as a model to study the role of cell-cell adhesion in bleb-driven single-cell migration in vivo. Utilizing a range of genetic, reverse genetic and mathematical tools, we define a previously unknown role for E-cadherin in confining bleb-type protrusions to the leading edge of the cell. We show that E-cadherin-mediated frictional forces impede the backwards flow of actomyosin-rich structures that define the domain where protrusions are preferentially generated. In this way, E-cadherin confines the bleb-forming region to a restricted area at the cell front and reinforces the front-rear axis of migrating cells. Accordingly, when E-cadherin activity is reduced, the bleb-forming area expands, thus compromising the directional persistence of the cells. The arrival of migratory cells at their targets relies on following precise routes within tissues. Here the authors demonstrate that the cell adhesion molecule E-cadherin can control the path of cell migration by confining the site where bleb-type protrusions form within the cell front. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|